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人巨噬细胞集落刺激因子(M-CSF)是一种能刺激巨噬细胞系细胞生长、增殖、分化的生长因子,其抗真菌活性越来越受到重视.利用小鼠化疗损伤模型研究家蚕表达的重组人巨噬细胞集落刺激因子(rhM-CSF)单独或与氟康唑联合应用时对白念珠菌感染的防治作用.一次性腹腔注射环磷酰胺200mg/kg,导致小鼠免疫功能下降.当小鼠外周血白细胞数降至最低时尾静脉注射白念珠菌.rhM-CSF治疗组分别采用每天10、50、250、500μg/kg4种剂量连续6d氟康唑每天300μg/kg,连续5d.记录各组小鼠的死亡天数和只数,计算平均存活天数和存活率.结果:rhM-CSF及rhM-CSF和氟康唑联合治疗组的小鼠存活率较空白组显著提高.250μg/kgrhM-CSF和氟康唑联合治疗组效果最好,小鼠存活率由空白组的14.3％提高至50.0％,存活小鼠肾脏白念珠菌菌落数也明显降低.表明家蚕表达的rhM-CSF对化疗损伤小鼠白念菌感染有明显的防治作用.为临床免疫功能低下病人感染白念珠菌的治疗提供了一种有效的新药.
Human macrophage colony-stimulating factor (M-CSF) is a kind of growth factor that can stimulate the growth, proliferation and differentiation of macrophage cell line, and its antifungal activity is more and more important.With the mouse model of chemical damage, Of recombinant human macrophage colony stimulating factor (rhM-CSF) alone or in combination with fluconazole on Candida albicans infection.Immunosuppression of mice immunosuppression caused by intraperitoneal injection of cyclophosphamide 200mg / kg Candida albicans was injected through the tail vein when the number of peripheral blood leukocytes in mice decreased to the lowest level.Optimum doses of 10, 50, 250 and 500 μg / kg of 4 doses of fluconazole (300 μg / kg) every day for 5 days were recorded in the rhM-CSF group The number of death days and the number of mice in each group were calculated and the average survival days and survival rate were calculated.Results: The survival rate of rhM-CSF, rhM-CSF and fluconazole group was significantly higher than that of the blank group.50μg / kg rhM- CSF and fluconazole combined treatment group was the best, the survival rate of mice increased from 14.3% to 50.0% in the blank group, the survival of mice kidney Candida albicans also significantly reduced the number of rhM-CSF expressed silkworm chemotherapy on the injury Mice infected with Candida albicans obvious Of the prevention and treatment of clinical immunocompromised patients infected with Candida albicans treatment provides an effective new drug.