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采用逆转录巢式ＰＣＲ技术检测了５１例ＨＣＣ患者的肝癌及癌周肝组织中ＨＣＶＲＮＡ正负链，同时以巢式ＰＣＲ技术检测了这些组织中的ＨＢＶＤＮＡ。结果，１１．８％（６／５１）检测出组织中ＨＣＶＲＮＡ正链；其中５例在癌周肝组织，２例在癌组织中检测出ＨＣＶＲＮＡ负链。由于负链ＲＮＡ为ＨＣＶ的复制中间体，不释放到细胞外，其检出为ＨＣＶ感染与ＨＣＣ关系的研究提供了进一步的病因证据。组织中ＨＢＶＤＮＡ检出率高达９２．２％（４７／５１），显示华南地区ＨＢＶ感染在ＨＣＣ的发生中起着特别重要的作用。
Reverse transcription-based nested PCR was used to detect the positive and negative HCVRNA in hepatocellular carcinoma (HCC) and peri-hepatic tissues of 51 patients with HCC. HBVDNA was detected by nested PCR in these tissues. As a result, 11.8% (6/51) of the HCV RNA positive strands were detected in the tissues, of which 5 cases were negative in the peritumoral liver tissue and 2 cases in the cancerous tissue. Since negative-stranded RNA is a replication intermediary of HCV that is not released into the extracellular domain, its detection provides further etiological evidence for the study of the relationship between HCV infection and HCC. The detection rate of HBVDNA in tissues was as high as 92.2% (47/51), indicating that HBV infection in southern China plays a particularly important role in the occurrence of HCC.