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目的 检测病毒性肝炎患者血清中SEN病毒D和H(SENV D、SENV H) ,并探讨其临床意义。方法 采用巢式聚合酶链反应法 (nPCR)检测甲、乙、丙、戊型肝炎和非甲～戊型肝炎患者血清中SENV D和SENV HDNA。结果 在 180例病毒性肝炎患者血清中 ,SENV D和SENV H检出率分别为 17.2 % (31 180 )和 5 .6 % (10 180 ) ,总检出率为 18.3% (33 180 )。甲、乙、丙、戊型肝炎患者的SENV D H检出率高于非甲～戊型肝炎患者。从甲、乙、丙、戊型肝炎和非甲～戊型肝炎患者中分离的SENV D H核苷酸序列 ,与SENV D H原型株比较 ,其同源性在 94 %以上。甲、乙、丙和戊型肝炎患者有无SENV D H合并感染 ,其血清生化学指标无明显差异。结论 SENV D H可能不是非甲～戊型肝炎的病原 ,甲、乙、丙和戊型肝炎患者合并感染SENV D H并不加重病情。
Objective To detect the serum SEN virus D and H (SENV D, SENV H) in patients with viral hepatitis and explore its clinical significance. Methods The serum levels of SENV D and SENV HDNA were detected by nested polymerase chain reaction (nPCR) in patients with hepatitis A, B, C, E and non-E hepatitis. Results The sera of 180 patients with viral hepatitis showed a positive rate of 17.2% (31 180) and 5 .6% (10 180) for SENV D and SENV H, respectively. The overall detection rate was 18.3% (33 180). The detection rate of SENV D H in patients with Grade A, B, C and C was higher than those in patients with Grade A and E hepatitis. The nucleotide sequence of SENV D H isolated from patients with hepatitis A, B, C, E, and non-A to E hepatitis was more than 94% homologous to the SENV D H prototype strain. There was no SENV D H co-infection in patients with A, B, C and E hepatitis, and there was no significant difference in serum biochemical indexes. Conclusions SENV D H may not be the cause of non-hepatitis E-E hepatitis. Patients with hepatitis A, B, C and E infection do not aggravate SENV D H infection.