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目的 :检测卵巢癌患者组织及外周血CD8~+T细胞中调节性T细胞(regulatory T cells,Treg)相关分子标志物的表达率,探讨其在卵巢癌发生发展中的临床意义。方法:用流式细胞术检测31例卵巢癌患者、31例卵巢良性肿瘤患者和31例健康对照者的外周血,以及14例卵巢癌组织和12例卵巢良性肿瘤组织CD8~+T细胞中Foxp3、CD25、CD28、CTLA-4及GITR的表达率。结果:Foxp3和CTLA-4在卵巢癌组织CD8~+T细胞中的表达率显著高于卵巢良性肿瘤组织(P<0.05);CD28在卵巢癌组织CD8~+T细胞中的表达率显著低于卵巢良性肿瘤组织(P<0.05)。卵巢癌患者外周血CD8+T细胞中Foxp3、CD25、CTLA-4的表达率显著高于卵巢良性肿瘤患者和健康对照者(P<0.05),CD28的表达率显著低于卵巢良性肿瘤患者和健康对照者(P<0.05)。结论:卵巢癌组织及外周血CD8~+Treg所占的比例显著高于卵巢良性肿瘤组及健康对照组,且CD8~+T细胞中Foxp3的表达率可能对了解卵巢癌进展状况有一定帮助。
OBJECTIVE: To detect the expression of regulatory T cells (Treg) -related molecular markers in ovarian cancer tissue and peripheral blood CD8 ~ + T cells and to explore its clinical significance in the development of ovarian cancer. Methods: The peripheral blood of 31 patients with ovarian cancer, 31 patients with benign ovarian tumor and 31 healthy controls were detected by flow cytometry, and the expressions of Foxp3 in CD8 + T cells in 14 ovarian cancer tissues and 12 benign ovarian tumor tissues , CD25, CD28, CTLA-4 and GITR expression rate. Results: The expression rates of Foxp3 and CTLA-4 in CD8 + T cells of ovarian cancer tissues were significantly higher than those in benign ovarian tissues (P <0.05). The expression rates of CD28 in CD8 + T cells of ovarian cancer tissues were significantly lower than Ovarian benign tumor tissue (P <0.05). The expression rates of Foxp3, CD25 and CTLA-4 in peripheral blood CD8 + T cells of patients with ovarian cancer were significantly higher than those of benign ovarian tumors and healthy controls (P <0.05), and the expression of CD28 was significantly lower than that of patients with benign ovarian tumors and healthy Control (P <0.05). Conclusion: The proportion of CD8 + Treg in ovarian cancer and peripheral blood is significantly higher than that in benign ovarian tumors and healthy controls. The expression of Foxp3 in CD8 + T cells may be helpful to understand the progress of ovarian cancer.