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目的：探讨原发性肝细胞癌（简称肝癌）细胞凋亡相关蛋白bcl－2和bax的表达情况及其意义。方法：采用免疫组化ABC法检测bcl－2和bax蛋白在66例人肝癌组织和24例人正常肝组织，以及3种人肝癌细胞系HCC－9204、SMMC－7721、HHCC和1种人正常肝细胞系QZG的表达情况，并分析肝癌组织的bcl－2和bax蛋白表达阳性率与其病理分级之间的关系。结果：bcl－2蛋白在正常肝组织中表达的阳性率只有4．2 ％（1／24），在肝癌组织中的阳性率为25．8 ％（17／66），二者具有显著性差异（P＜0．05）。bax蛋白在正常肝组织中表达的阳性率为70．8 ％（17／24），在肝癌组织中的阳性率为43．9 ％（29／66），二者也具有显著性差异（P＜0．05）。肝癌组织的bcl－2和bax蛋白表达阳性率与其病理分级之间均未见明显的相关性。在正常肝和肝癌组织中，bcl－2蛋白的阳性率均明显低于bax蛋白（P＜0．01，P＜0．05）。在正常肝细胞系QZG中未见明显的bcl－2阳性信号，bax蛋白的阳性细胞计数率为61．0％。肝癌细胞系SMMC－7721和HHCC的bcl－2蛋白阳性细胞计数率分别为14．3％和11．8％，bax蛋白阳性细胞计数率分别为58．3％和47．1％。肝癌细胞系HCC－9204中未见明显的bcl－2阳性信号，bax蛋白阳性细胞计数率为43．2％。结论：与正常肝组?
Objective: To investigate the expression and significance of apoptosis-related proteins bcl-2 and bax in primary hepatocellular carcinoma (HCC). METHODS: Immunohistochemical ABC method was used to detect bcl-2 and bax protein in 66 cases of human hepatocellular carcinoma and 24 cases of normal human liver tissue, and 3 types of human hepatocellular carcinoma cell lines HCC-9204, SMMC-7721, HHCC and 1 normal The expression of QZG in hepatic cell lines was analyzed, and the relationship between the positive rate of bcl-2 and bax protein expression and the histological grade was analyzed. Results: The positive rate of bcl-2 protein expression in normal liver tissue was only 4.2% (1/24). The positive rate in bcl-2 protein was 25.8% (17/66). There was a significant difference between the two. (P<0.05). The positive rate of bax protein expression in normal liver tissues was 70.8 % (17/24), and the positive rate in liver cancer tissues was 43.9 % (29/66). There was also a significant difference between the two (P< 0.05). There was no significant correlation between the positive rate of bcl-2 and bax protein expression and the histological grade in HCC. In normal liver and liver cancer tissues, the positive rate of bcl-2 protein was significantly lower than that of bax protein (P<0.01, P<0.05). No obvious bcl-2 positive signal was found in the normal hepatocyte cell line QZG, and the bax protein positive cell count rate was 61.0%. The positive rates of bcl-2 protein in SMMC-7721 and HHCC were 14.3% and 11.8% respectively, and the bax protein positive cells were 58.3% and 47.1%, respectively. There was no obvious bcl-2 positive signal in HCC-9204 cell line, and the bax protein positive cell count rate was 43.2%. Conclusion: With normal liver group?