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目的研究原癌基因Bmi-1和hTERT的表达变化及其与细胞凋亡的关系,探讨它们在乳腺癌发生、发展过程中的作用。方法采用MTT法检测乳腺癌细胞的增殖抑制情况,并确定所选用的药物浓度。RT-PCR、Western Blot检测Bmi-1、hTERT的mRNA及蛋白的表达情况;TUNEL、流式细胞技术检测乳腺癌细胞的凋亡情况。结果 5-FU对乳腺癌细胞有明显抑制作用,各实验组与对照组OD值的差异有统计学意义(P<0.05)。用5-FU干预后,实验组Bmi-1和hTERT的mRNA及蛋白表达水平均降低,细胞凋亡指数增加,与对照组相比差异显著有统计学意义(P<0.05),经统计学分析两者呈正相关(P<0.05),且两者的表达与凋亡呈负相关(P<0.05)。结论 Bmi-1和hTERT的表达下调可促进乳腺癌细胞凋亡。
Objective To study the expression changes of Bmi-1 and hTERT and its relationship with apoptosis and to explore the role of Bmi-1 and hTERT in the occurrence and development of breast cancer. Methods MTT assay was used to detect the proliferation inhibition of breast cancer cells and to determine the concentration of selected drugs. The mRNA and protein expression of Bmi-1 and hTERT were detected by RT-PCR and Western Blot. TUNEL and flow cytometry were used to detect the apoptosis of breast cancer cells. Results 5-FU had significant inhibitory effect on breast cancer cells, and there was significant difference in OD value between experimental group and control group (P <0.05). After intervention with 5-FU, the mRNA and protein expression of Bmi-1 and hTERT in the experimental group decreased and the apoptotic index increased, which was significantly different from the control group (P <0.05). After statistical analysis (P <0.05), and both of them were negatively correlated with apoptosis (P <0.05). Conclusion The down-regulation of Bmi-1 and hTERT may promote the apoptosis of breast cancer cells.