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目的:探讨纳洛酮对丙泊酚致小鼠学习记忆障碍以及催眠作用的影响。方法:小鼠腹腔注射不同剂量的丙泊酚(10,100 mg·kg-1)建立模型,跳台、避暗实验中观察不同剂量的纳洛酮sc对小鼠跳台潜伏期(stepdown latency,SDL)、步入潜伏期(step through latency,STL)和错误次数的影响;催醒实验中观察不同剂量的纳洛酮皮下注射(subcutaneously,sc)对小鼠睡眠潜伏期和持续时间(sleeping time,ST)的影响。结果:跳台、避暗实验中,用药后第1 d,纳洛酮0.5 mg·kg-1sc能够延长丙泊酚所致记忆障碍小鼠的SDL、STL,减少错误次数(P<0.05;P<0.01),用药后第2、3、4 d,各组SDL、STL和错误次数的差异均无显著性(P>0.05);催醒实验中,纳洛酮0.5 mg·kg-1sc对丙泊酚催眠小鼠的潜伏期和ST没有明显影响(P>0.05),纳洛酮1.2 mg·kg-1sc能够延长丙泊酚催眠小鼠的潜伏期,缩短其ST(P<0.05;P<0.01)。结论:纳洛酮可以改善丙泊酚所致的学习记忆障碍,可以减轻丙泊酚的催眠作用。
Objective: To investigate the effects of naloxone on learning and memory impairments and hypnotic effects induced by propofol in mice. METHODS: Mice were injected intraperitoneally with different doses of propofol (10,100 mg · kg-1) to establish a model. The mice were challenged with different doses of naloxone sc against step-down latency (SDL) The effects of different doses of naloxone subcutaneously (sc) on the sleep latency and the duration of sleep were observed in wakefulness experiments. Results: In the step-down and dark-avoidance experiments, naloxone 0.5 mg · kg-1sc could prolong the SDL and STL of mice with memory impairment induced by propofol and reduce the number of errors (P <0.05; P < 0.01). There were no significant differences in the number of SDL, STL and errors between the two groups on the 2nd, 3rd, 4th day after drug administration (P> 0.05). In the wake-up experiment, naloxone 0.5 mg · kg- Phenol-hypnotic mice latency and ST had no significant effect (P> 0.05), naloxone 1.2 mg · kg-1sc can prolong the incubation period of propofol hypnotic mice and shorten its ST (P <0.05; P <0.01). Conclusion: Naloxone can improve the learning and memory impairment caused by propofol and reduce the hypnotic effect of propofol.