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本文报道晶体蛋白非酶促糖基化的抑制与中药预防大鼠老年性白内障的实验研究结果。在体外研究系统中,以阿斯匹林(Aspirin)作为抑制剂,实验结果表明Aspirin可明显抑制糖基化作用,抑制率与Aspirin浓度和保温时间成正相关,20mMAspirin在保温第15天的最大抑制率为40％。糖基化水平降低,可防止硫氢基氧化并减少高分子蛋白聚合物的生成。体内实验,用抗衰老中药喂饲16月龄大鼠,连续6个月,比较药物组与对照组晶体蛋白糖基化水平与白内障发生率的关系。结果表明,药物组晶体蛋白糖基化水平较同龄对照组明显降低,肝脏超氧化物歧化酶活性较同龄对照组升高,上述二指标均与4月龄年轻大鼠相似。药物组白内障发生率较同龄对照组降低约50％,P<0.005。上述结果预示,用抗衰老中药预防与延缓大鼠老年性白内障的可能性。
This article reports the inhibition of crystalline protein non-enzymatic glycosylation and the experimental study of traditional Chinese medicine to prevent senile cataract in rats. In the in vitro research system, Aspirin was used as an inhibitor. The results showed that Aspirin can significantly inhibit glycosylation. The inhibition rate is positively related to Aspirin concentration and incubation time, and the maximum inhibition of 20mMAspirin on the 15th day of incubation. The rate is 40%. Reduced levels of glycosylation can prevent sulfhydryl oxidation and reduce polymer protein production. In vivo experiments, rats aged 16 months were fed with anti-aging herbs for 6 consecutive months. The relationship between the level of crystal protein glycosylation and the incidence of cataract was compared between the drug group and the control group. The results showed that the glycosylation level of crystal protein in the drug group was significantly lower than that in the control group of the same age, and the activity of superoxide dismutase in the liver was higher than that in the control group of the same age. Both of these parameters were similar to those of young rats aged 4 months. The incidence of cataract in the drug group was about 50% lower than that in the same age control group, P<0.005. The above results indicate that the use of anti-aging herbs prevents and delays the possibility of senile cataract in rats.