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目的:检测胃癌组织中的端粒酶活性和基质金属蛋白酶-7(MMP-7)的表达,并探讨其临床意义。方法:在49例胃癌组织(转移30例,未转移19例)和20例癌前病变组织中,采用RT-PCR法检测MMP-7 mRNA,TRAP法检测端粒酶活性。结果:49例胃癌组织中,MMP-7 mRNA及端粒酶阳性表达者分别为28例和32例,其中转移组表达分别为21例和20例;20例癌前病变组织中MMP-7、端粒酶表达分别为4例和8例。胃癌组联合检测均阳性表达者19例,均阴性者8例;癌前病变组联合检测均阳性者仅3例,均阴性者11例。而MMP-7 mRNA表达在性别、年龄间差异无统计学意义(P>0.05);2组在病理分级中,Ⅰ、Ⅱ级的表达明显低于Ⅲ级(P<0.01);侵及浆膜层者明显高于未侵及者(P<0.01)。端粒酶在性别、年龄和病理分级以及是否侵及浆膜层间差异均无统计学意义(P>0.05)。结论:MMP-7、端粒酶与胃癌的发生、发展可能有关,高表达者胃癌转移的风险可能较高。联合检测较单一指标的检测率高。
OBJECTIVE: To detect the telomerase activity and the expression of matrix metalloproteinase-7 (MMP-7) in gastric cancer tissue and to explore its clinical significance. Methods: MMP-7 mRNA was detected by RT-PCR in 49 cases of gastric carcinoma (30 cases without metastasis and 19 cases without metastasis) and 20 cases with precancerous lesions. The telomerase activity was detected by TRAP assay. Results: In 49 cases of gastric carcinoma, the expression of MMP-7 mRNA and telomerase were 28 cases and 32 cases respectively, of which the expression in metastasis group was 21 cases and 20 cases respectively. In 20 cases of precancerous lesions, the expression of MMP-7, Telomerase expression were 4 cases and 8 cases respectively. 19 cases were detected positive in gastric cancer group, all of them were negative in 8 cases. Only 3 cases were detected positive in precancerous lesions group, 11 cases were negative. There was no significant difference in gender and age between the expression of MMP-7 mRNA (P> 0.05). In the pathological grade, the expression of MMP-7 mRNA in grade Ⅰ and Ⅱ was significantly lower than that in grade Ⅲ (P <0.01) The levels were significantly higher than those without invasion (P <0.01). There was no significant difference in telomerase between gender, age, pathological grade and invasion of serosa (P> 0.05). Conclusion: The expression of MMP-7 and telomerase may be related to the occurrence and development of gastric cancer, and the high risk of gastric cancer metastasis may be high. Joint detection of a single indicator of high detection rate.