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目的检测食物中毒样本中的产气荚膜梭菌,为同类事件检测和判定提供依据。方法利用快速显微镜便检锁定检测方向;利用TSC培养基厌氧培养产气荚膜梭菌;利用产气荚膜梭菌α、β、ε、ι毒素和CPE、β2肠毒素PCR扩增检测产气荚膜梭菌所含肠毒素类型。结果在食品留样、环境涂抹拭子和患者粪便标本中共检出产气荚膜梭菌阳性样本8例。1份食品样本和5份患者粪便样本中分离株毒素携带情况完全一致,为含有α、β和肠毒素β2的C型产气荚膜梭菌;从环境试子中分离的菌株6种毒素均为阴性。结论此次事件是一起由C型产气荚膜梭菌毒素导致的细菌性食物中毒,食品与环境之间存在产气荚膜梭菌的交叉污染。作为重要食物中毒致病因的产气荚膜梭菌的监测与检测工作有待开展与标准化。
Objective To detect Clostridium perfringens in food poisoning samples and provide basis for the detection and determination of similar events. Methods Rapid detection microscope was used to test the detection direction. Clostridium perfringens was cultured anaerobically in TSC culture medium. Clostridium perfringens α, β, ε, ι toxin and CPE, β2 enterotoxin PCR amplification Clostridium perfringens contains the type of enterotoxin. Results 8 samples of Clostridium perfringens positive samples were detected in food sample, environmental smear swab and patient stool samples. 1 food samples and 5 patients stool samples isolated toxins carried exactly the same, containing α, β and enterotoxin β2 Clostridium perfringens type C; isolated from the environmental test strains were 6 toxins Negative. Conclusion The incident was a bacterial food poisoning caused by Clostridium perfringens type C, with cross-contamination of Clostridium perfringens between food and the environment. As an important cause of food poisoning Clostridium perfringens monitoring and testing work to be done and standardization.