论文部分内容阅读
目的:探讨白细胞介素-12(IL-12)、干扰素γ(IFNγ)、IL-4、IL-10在肾综合征出血热(HFRS)发病过程中的变化及其致病意义。方法:27例HFRS患者按病情划分为2组,轻症组18例,重症组9例;按病期采血,分别用EILSA方法检测IL-12、IFNγ,用放射免疫法检测IL-4、IL-10,并同步检测血象和肾功能。结果:从发热期到多尿期血清IL-12均显著高于对照组水平(P<0.01),恢复期则趋于正常;各病期IFNγ含量均见升高,但以发热期和低血压少尿期为最著,轻、重两组间记显著差异,重症组IFNγ水平高于轻症组。各期IL-4均在正常范围,IL-10则高于正常水平,但升高幅度不及IFNγ。IFNγ的变化曲线与BUN相平行,而与血小板的变化曲线相反。结论:IL-12、INFγ等细胞因子的过多释放参与了HFRS的发病机制,是造成全身炎性反应综合征(SIRS)的重要因素之一。
Objective: To investigate the changes of IL-12, IFNγ, IL-4 and IL-10 in the pathogenesis of hemorrhagic fever with renal syndrome (HFRS) and their pathogenic significance. Methods: Twenty-seven patients with HFRS were divided into two groups according to their disease: 18 cases in mild group and 9 cases in severe group. Blood samples were collected from patients with HFRS. IL-12 and IFNγ were detected by EILSA and IL-4 and IL- -10, and simultaneous detection of blood and kidney function. Results: Serum IL-12 levels from fever to polyuria were significantly higher than those in the control group (P <0.01), while the recovery period tended to be normal. The levels of IFN-γ in all the stages were increased, but in the period of fever and hypotension Oliguria was the most significant, light and heavy significant differences between the two groups, severe group IFNγ levels were higher than the mild group. The levels of IL-4 in the normal range, IL-10 was higher than the normal level, but not as high as IFNγ. IFNγ curve and BUN parallel to the line, and the curve of the opposite platelet. CONCLUSION: Excessive release of cytokines, such as IL-12 and INFγ, is involved in the pathogenesis of HFRS and is one of the important factors contributing to systemic inflammatory response syndrome (SIRS).