合成了试剂二安替比林对羟基苯乙烯基甲烷 (DApHVM) ,研究了它与V(Ⅴ )的显色反应。在H3PO4介质中 ,Mn(Ⅱ )和Tween 2 0协同作用下 ,DApHVM与V(Ⅴ )生成红色产物 ,λmax为 540nm ,ε=1 0 3× 1 0 6 L·mol-1 ·cm-1 ,V(Ⅴ )的质量浓度在 0～ 50 μg/L内符合比尔定律。方法用于中草药及钢样中V(Ⅴ )的测定 ,结果与原子吸收法一致 ,加标回收率在 96%～ 1 0 5 % ,5次测定结果的RSD <3 5 %。 A reagent diantipyryl p-hydroxy-styrylmethane (DApHVM) was synthesized and its color reaction with V (Ⅴ) was studied. In H3PO4 media, DApHVM and V (Ⅴ) produced red product with a maxmax of 540 nm and ε = 103 × 106 L · mol-1 · cm-1 under the synergistic effect of Mn (Ⅱ) and Tween 20, Beer ’s law is obeyed in the range of 0 ~ 50 μg / L for V (V). The method was applied to the determination of V (Ⅴ) in Chinese herbal medicines and steel samples. The results were in good agreement with those of atomic absorption spectrometry. The recoveries were between 96% and 105%. The RSDs of 5 determinations were less than 35%.