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目的观察适量运动和甲状腺激素对大鼠肝脏H_2O_2含量和抗氧化能力的影响。方法将64只Wistar大鼠随机分为8组:对照组(C)、轻度甲亢组(T_1)、重度甲亢组(T_2)、甲减组(J)、运动对照组(E)、轻度甲亢运动组(ET-1)、重度甲亢运动组(ET-2)、甲减运动组(EJ)。C组生理盐水灌胃,T_1和T_2组分别给予左旋甲状腺素钠100和200μg/d灌胃,J组给予甲巯咪唑10 mg/d灌胃,运动为负重6%体重游泳训练30 min/d。喂养14 d后,检测肝组织匀浆中的H_2O_2含量、谷胱甘肽过氧化酶(GPx)活力、超氧化物歧化酶(SOD)活力以及丙二醛(MDA)含量。结果非运动条件下,甲亢组与对照组比较,H_2O_2、GPx、SOD和MDA水平均增高,差异有统计学意义(P<0.05);甲减组与对照组比较,H_2O_2、GPx、SOD和MDA水平均降低,差异有统计学意义(P<0.05);甲亢运动组和甲减运动组与运动对照组比较,H_2O_2、GPx、SOD和MDA水平均增高,差异有统计学意义(P<0.05);甲状腺激素水平异常的运动组与对应非运动组比较,H_2O_2、GPx、SOD和MDA水平均增高,差异有统计学意义(P<0.05);而甲状腺激素水平正常的运动对照组与对应非运动组比较,MDA差异无统计学意义(P>0.05)。结论运动和甲亢均可诱发大鼠肝脏H_2O_2含量和抗氧化酶活力增高,二者有一定的协同作用;甲亢、甲亢合并运动和甲减合并运动均会诱发失代偿性氧化损伤,单纯的适量运动不会诱发氧化损伤。
Objective To observe the effect of moderate exercise and thyroid hormones on H 2 O 2 content and antioxidant capacity in rat liver. Methods Sixty - four Wistar rats were randomly divided into 8 groups: control group (C), mild hyperthyroidism group (T 1), severe hyperthyroidism group (T 2), hypothyroidism group (J), exercise control group (E) Hyperthyroidism group (ET-1), severe hyperthyroidism group (ET-2) and hypothyroidism group (EJ). Rats in group C were given intragastric administration of normal saline. Rats in groups T_1 and T_2 were treated with 100 and 200 μg / L of levothyroxine respectively. Group J was given 10 mg / d of methimazole orally, and exercise was 6% of weight-bearing swimming training for 30 min / d . H 2 O 2 content, glutathione peroxidase (GPx) activity, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in liver homogenates were detected after 14 d of feeding. Results Compared with the control group, the levels of H_2O_2, GPx, SOD and MDA in hyperthyroid group were significantly increased (P <0.05), while the levels of H_2O_2, GPx, SOD and MDA in the hypothyroid group were significantly lower than those in the control group (P <0.05). The levels of H_2O_2, GPx, SOD and MDA in hyperthyroidism group and hypothyroidism group were higher than those in the control group (P <0.05), the difference was statistically significant ; The level of H_2O_2, GPx, SOD and MDA in the exercise group with abnormal thyroid hormone level were significantly higher than those in the corresponding non-exercise group (P <0.05), while those with normal thyroid hormone level and the corresponding non-exercise group There was no significant difference in MDA between the two groups (P> 0.05). Conclusion Both exercise and hyperthyroidism can induce H 2 O 2 content and antioxidant enzyme activity in the liver of rats with increased synergism. Both hyperthyroidism and hyperthyroidism combined exercise and hypothyroidism and exercise can induce decompensated oxidative damage. Exercise does not induce oxidative damage.