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为研究IL-22与NOD小鼠自发性胚胎吸收率增高的关系,多次腹腔注射中和抗体抑制IL-22,采用流式细胞术检测子宫CD4+IL-22+细胞百分率,并观察NOD×C57BL/6小鼠交配组合中孕鼠胚胎吸收率的变化。结果显示,NOD×C57BL/6孕鼠子宫CD4+IL-22+细胞百分率显著高于BALB/c×C57BL/6孕鼠(52.4%±2.1%与14.3%±4.4%,P<0.01)。多次腹腔注射中和抗体可显著降低NOD×C57BL/6孕鼠子宫内CD4+IL-22+细胞数量(18.7%±2.8%;与未抑制组相比,P<0.01),并可显著降低这些小鼠孕12.5 d的胚胎吸收率(4.8%±2.0%与19.1%±5.1%,P<0.05)。线性相关-回归分析显示,胚胎吸收率与子宫CD4+IL-22+细胞百分率呈正相关。提示妊娠子宫内CD4+IL-22+细胞相对数量过多是妊娠不利因素,可能是造成NOD×C57BL/6孕鼠胚胎吸收率增高的原因之一。
In order to study the relationship between IL-22 and spontaneous embryo uptake in NOD mice, multiple intraperitoneal injection of neutralizing antibody inhibited IL-22, and the percentage of CD4 + IL-22 + cells in uterus was detected by flow cytometry. Changes of Embryo Absorption Rate in Pregnant C57BL / 6 Mice during Intercrossing. The results showed that the percentage of CD4 + IL-22 + cells in uterus of NOD × C57BL / 6 pregnant mice was significantly higher than that of BALB / c × C57BL / 6 pregnant mice (52.4% ± 2.1% vs 14.3% ± 4.4%, P <0.01). Repeated intraperitoneal injection of neutralizing antibody significantly reduced the number of utero CD4 + IL-22 + cells (18.7% ± 2.8%) in NOD × C57BL / 6 pregnant mice compared with those in non-inhibited group (P <0.01) Embryos uptake by these mice at 12.5 d (4.8% ± 2.0% vs 19.1% ± 5.1%, P <0.05). Linear correlation - regression analysis showed that embryonic absorption rate and uterine CD4 + IL-22 + cell percentage was positively correlated. It is suggested that the relative number of CD4 + IL-22 + cells in utero during pregnancy is an unfavorable factor in pregnancy and may be one of the reasons for the increased embryo uptake in NOD × C57BL / 6 pregnant mice.