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用致病力不同的甘薯青枯菌株(Pseudom onassolanacearum )进行毒素产生条件研究。试验结果表明,不同pH值、培养天数、菌株浓度及不同致病力的菌株所制备的毒素毒力差异达显著和极显著,但不同培养温度所制备的毒素毒力差异不显著。用Lotus1-2-3 电脑软件综合分析以上产毒条件对所制备的甘薯青枯菌毒素的毒力影响程度,计算出强、弱和混合菌株产生毒素的3 个最优回归方程。分别用Basic和Fortran 语言编写程序,求出强、弱和强弱混合的甘薯青枯病菌产生毒素的3 个最优模式为:①强菌株:pH= 7,菌株浓度108 菌细胞/m l,培养天数3 d。②弱菌株:pH= 7,菌株浓度1012菌细胞/m l,培养天数2 d。③混合菌株:pH= 7,菌株浓度1014菌细胞/m l,培养天数1 d。培养温度均为25～35℃
The toxin production conditions were studied with Pseudomonas parvulanacearum with different pathogenicity. The results showed that the differences of the virulence of toxins prepared by different pH value, culture days, strain concentration and different virulence strains were significant and very significant, but the difference of the virulence of toxins prepared at different culture temperature was not significant. Using Lotus1-2-3 computer software to comprehensively analyze the degree of virulence of the toxin production to R. solanacearum toxin produced by the above production conditions, three optimal regression equations for producing toxins from strong and weak and mixed strains were calculated. The programs were prepared in Basic and Fortran languages, respectively. The three optimal models for producing toxins from the mixed bacterial strains of strong, weak and strong were as follows: ① Strong strain: pH = 7, strain at 108 cells / ml, culture Number of days 3 d. ② weak strains: pH = 7, the strain concentration of 1012 cells / ml, culture days 2 d. ③ mixed strains: pH = 7, the strain concentration of 1014 cells / ml, culture days 1 d. Culture temperature is 25 ~ 35 ℃