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目的研究分析昆明市2006年注射吸毒者中艾滋病病毒-1型(HIV-1)的基因亚型流行情况,为更清楚地掌握昆明市HIV流行模式,制订抗病毒治疗方案和疫苗研发提供参考数据。方法采用“一对一”的问卷调查,收集基本人口学特征及HIV感染危险行为信息,采集血样,利用套式聚合酶链反应(Nested-PCR)对HIV前病毒的gag基因进行扩增,对扩增产物进行纯化、测序,通过构建系统进化树和在线比对来判定序列所属基因亚型。结果62名研究对象所感染HIV-1gag基因被成功扩增、测序、判定基因亚型。其中1人所感染的HIV-1为CRF01-AE重组型,其余均为CRF07-BC重组型或CRF08-BC重组型。12名对象除注射海洛因外,还发生过商业性行为,感染CRF01-AE的对象就是发生过商业性行为的注射吸毒者。结论2006年发现的HIV-1阳性注射吸毒者中,CRF07-BC、CRF08-BC重组型毒株占据了绝对优势,同时存在CRF01-AE的流行。
Objective To study the prevalence of HIV-1 genotypes among injecting drug users in Kunming in 2006, and to provide reference data for more clearly understanding of HIV epidemic patterns in Kunming and formulating antiviral treatment programs and vaccine research and development . Methods A one-to-one questionnaire was conducted to collect basic demographic characteristics and risk behaviors of HIV infection. Blood samples were collected and the gag gene of HIV provirus was amplified by nested-polymerase chain reaction (Nested-PCR) The PCR products were purified and sequenced. The phylogenetic tree and online alignment were used to determine the genotypes of the sequences. Results The 62 HIV-1 gag genes infected were successfully amplified, sequenced and the gene subtypes were determined. Among them, 1 was HIV-1 CRF01-AE recombinant, the rest were CRF07-BC recombinant or CRF08-BC recombinant. 12 subjects in addition to injecting heroin, but also the occurrence of commercial sex, CRF01-AE infected with the object is the occurrence of commercial practice of injecting drug users. Conclusions CRF07-BC and CRF08-BC recombinant strains occupy an absolute advantage among the HIV-1 positive injecting drug users found in 2006, and the prevalence of CRF01-AE exists at the same time.