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目的 :观察东亚钳蝎毒 (BMK)对 K 5 6 2 /ADM细胞多药耐药性 (MDR)的逆转作用。方法 :MTT法 ,Hoechst33342和 PI荧光染色 ,荧光分光光度法和流式细胞分光光度术。结果 :K5 6 2 /ADM细胞对阿霉素 (ADM)具有明显的抗性 ,与 K5 6 2细胞相比 ,抗性倍数约为 33倍 ,而两者对BMK的 IC50 接近 ,差异无显著性 (P>0 .0 5 ) ;低毒剂量 BMK(10 .0 μg/m L,5 0 .0 μg/m L)与 ADM(4.0μg/m L)联合应用 ,可显著增强 ADM对该细胞的生长抑制和诱导凋亡作用 ,升高细胞内 ADM的浓度 ,降低该细胞对 ADM的 IC50 ,使该细胞对 ADM的抗性有数倍逆转。结论 :BMK可能是一种有效的MDR逆转剂 ,且与诱导细胞凋亡密切相关
Objective: To observe the reversal of multidrug resistance (MDR) in K562/ADM cells by BMK. Methods: MTT assay, Hoechst33342 and PI fluorescence staining, fluorescence spectrophotometry and flow cytometry. RESULTS: K562/ADM cells showed obvious resistance to adriamycin (ADM). Compared with K562 cells, the fold-resistance was about 33-fold, and the IC50 of the two were close to BMK. There was no significant difference between the two groups. (P>0.05); low toxic dose of BMK (10. 0 μg/m L, 5.0 μg/m L) combined with ADM (4.0 μg/m L) can significantly enhance ADM on the cells. The growth inhibition and induction of apoptosis, increased intracellular ADM concentrations, decreased the IC50 of the cells to ADM, so that the resistance of the cells to ADM several times reversed. Conclusion: BMK may be an effective MDR reversal agent and is closely related to the induction of apoptosis.