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油菜菌核病(Sclerotinia sclerotiorum)是油菜生产上最重要的病害之一,其致病性可能来源于基因水平转移(Horizontal gene transfer,HGT).为认识其致病原理和寻找新的真菌抑制剂的靶点,首先通过BLASTp发现其基因XM_001585458.1编码蛋白XP_001585508.1与细菌比对结果中出现低E值3.23e-109和高SCORE值436,暗示存在HGT现象;进一步通过系统进化树的建立,发现该蛋白在进化分枝上更接近于细菌中由Streptomyces sp.C的NZ_CM000832.1基因编码的蛋白ZP_07291173;同时核苷酸组成分析也发现该基因与油菜菌核病菌基因组的碱基组成有较大差别,GC含量提高了14.95%.这些结果证明了XM_001585458.1的确存在基因水平转移事件.结构分析和COG蛋白功能分类显示该HGT序列编码蛋白XP_001585508.1具有FA58C_3(Coagulation factors 5/8 type C domain)、Kelch repeat type 1、Galactose-binding domain-like、Galactose oxidase/kelch,beta-propeller等保守结构域,应为一个膜蛋白并参与多糖代谢,推测该水平转移基因与S.sclerotiorum在侵染植物时进行细胞壁水解和致病性有关.
Sclerotinia sclerotiorum is one of the most important diseases in rapeseed production, and its pathogenicity may originate from Horizontal Gene Transfer (HGT) .In order to understand its pathogenicity and find new fungal inhibitors , The low E value of 3.23e-109 and the high SCORE value of 436, indicating the presence of HGT, were found by BLASTp. The results of phylogenetic tree were further confirmed by BLASTp, which showed that XM_001585458.1 encoded protein XP_001585508.1 showed high H / And found that the protein was closer to the ZP_07291173 protein encoded by the NZ_CM000832.1 gene of Streptomyces sp. C in the phylogenetic tree. The nucleotide composition analysis also showed that the base composition of the gene and the genome of B. sclerotiorum The results showed that XM_001585458.1 did indeed have the gene transfer events.The structural analysis and functional classification of COG protein showed that the HGT sequence encoding protein XP_001585508.1 had the function of FA58C_3 (Coagulation factors 5/8 type C domain, Kelch repeat type 1, Galactose-binding domain-like, Galactose oxidase / kelch, beta-propeller and other conservative domains should be A membrane protein involved in polysaccharide metabolism, suggesting that the horizontal transfer gene is involved in cell wall hydrolysis and pathogenicity of S. sclerotiorum in infecting plants.