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目的 研究中药MBC提取物在体外对K5 62 /ADM逆转作用及机制。方法 应用MTT法测定应用MBC前后ADM对K5 62 /ADM耐药细胞株的IC50 ,计算其逆转倍数。应用流式细胞仪技术测定应用MBC前后细胞内Rh12 3浓度。结果 8× 10 -1 μg/mlMBC能增加ADM对K5 62 /ADM的细胞毒作用 ,逆转倍数 6 2 8倍 ,应用MBC后K5 62 /ADM细胞内Rh12 3浓度增加。结论 MBC可提高ADM对耐药细胞K5 62 /ADM的杀伤作用 ,部分逆转了K5 62 /ADM细胞的耐药
Objective To study the reversal effect and mechanism of MBC extract of Chinese medicine on K5 62 /ADM in vitro. Methods The IC50 of ADM on K562/ADM cell line before and after MBC application was determined by MTT method, and the fold reverse ratio was calculated. Flow cytometry was used to determine the intracellular concentration of Rh12 3 before and after the application of MBC. Results 8×10 -1 μg/ml MBC could increase the cytotoxicity of ADM on K562/ADM, and the reversal fold was 628 times. The concentration of Rh12 3 in K5 62 /ADM cells increased after MBC application. Conclusion MBC can enhance the killing effect of ADM on drug-resistant cell K5 62/ADM, partially reversed the resistance of K5 62 /ADM cells.