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目的探讨Real time PCR在社区获得性肺炎患者肺炎支原体检测中的临床应用价值。方法选择100例社区获得性肺炎患者为研究对象,获取急性期和恢复期咽拭子标本以及急性期痰标本,采用Real time PCR对肺炎支原体进行检测和定量。结果咽拭子标本急性期检出率为75.5%,恢复期为47.3%,急性期与恢复期比较,差异有统计学意义(P<0.05)。急性期痰液标本中肺炎支原体阳性率为100%,其中肺炎支原体含量的检出率与咽拭子相比差异无统计学意义。肺炎支原体感染与其他细菌感染相比,只有血沉有统计学差异(P<0.05),而白细胞数目和肺CT改变均无统计学差异。结论痰标本采用Real time PCR方法是检测肺炎支原体的理想方法,若无痰时,可考虑用咽拭子代替。而其他临床指标不能作为诊断肺炎支原体感染的唯一依据。
Objective To explore the clinical value of Real time PCR in the detection of Mycoplasma pneumoniae in patients with community-acquired pneumonia. Methods 100 patients with community-acquired pneumonia were selected as research objects. Throat swabs and acute sputa specimens were collected during acute and convalescent stages. Mycoplasma pneumoniae was detected and quantitated by Real time PCR. Results The positive rate of throat swab was 75.5% in acute phase and 47.3% in convalescence phase. There was significant difference between acute phase and convalescent phase (P <0.05). The positive rate of Mycoplasma pneumoniae was 100% in acute sputum samples, and the detection rate of Mycoplasma pneumoniae was not significantly different from that of throat swabs. Mycoplasma pneumoniae infection compared with other bacterial infections, only the ESR were statistically significant (P <0.05), while the number of leukocytes and lung CT changes were not statistically different. Conclusion Real time PCR method is an ideal method for the detection of Mycoplasma pneumoniae in sputum. If there is no sputum, throat swab can be considered instead. While other clinical indicators can not be used as the only basis for the diagnosis of Mycoplasma pneumoniae infection.