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叶片是光合作用的主要器官,适度卷曲有利于改善群体光照,提高光能利用率,因此,发掘和研究叶片发育相关基因是改良株型和植物生长发育研究的重要基础工作。本研究报道了一个新的水稻稳定遗传卷叶突变体rolled leaf 28(rl28),与野生型相比,rl28从拔节期起叶片开始沿中轴脉向内侧卷曲,叶片的卷曲度均极显著高于野生型,且叶夹角也不同程度小于野生型。扫描电镜及石蜡切片观察表明,rl28叶片单位面积气孔数、气孔导度显著高于野生型,蒸腾速率极显著高于野生型,rl28中脉增大及临近的2个泡状细胞数量减少。遗传分析表明该突变性状受1对隐性核基因控制,RL28基因被定位在第5染色体标记5-43和5-34之间,物理距离为90 kb。本研究将为RL28基因的图位克隆及功能研究奠定基础。
Leaf is the main organ of photosynthesis. Appropriate curl helps to improve population illumination and improve the utilization of light energy. Therefore, to explore and study leaf development-related genes is an important basic work to improve plant type and plant growth and development. In this study, a new rice leaf rolling mutant 28 (rl28) was reported. Compared with the wild type, rl28 curled inwardly along the medial malleolus from the jointing stage, and the curl of the leaves was extremely high In the wild type, and the angle between the leaves is also less than the wild type. Scanning electron microscopy and paraffin sections showed that the number of stomata and stomatal conductance per unit area of rl28 was significantly higher than that of wild type and the transpiration rate of rl28 was significantly higher than that of wild type. The number of rl28 midrib and the number of 2 nearby blister cells decreased. Genetic analysis showed that the mutant trait was controlled by a pair of recessive nuclear genes. The RL28 gene was mapped between chromosomes 5-43 and 5-34 on chromosome 5 with a physical distance of 90 kb. This study will lay the foundation for the mapping of RL28 gene and its function.