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目的:明确头花蓼抗菌作用的物质基础。方法:采用D101大孔树脂和MCI柱层析技术分段制备头花蓼各组分;采用UHPLC-UV技术建立头花蓼各组分的图谱,并通过对照品对主要色谱峰进行归属指认;采用均匀设计,将获得的组分配制成峰组成各不相同的样品;通过考察各样品对泌尿系统主要感染菌大肠埃希菌的最低抑菌浓度(MIC)确定它们的抑菌活性;运用灰色关联度分析法对1/MIC与色谱峰峰面积进行相关性分析,明确与抑菌活性相关的主要色谱峰,确定头花蓼抑菌作用的物质基础。结果:色谱峰没食子酸(1)、表儿茶素(6)、儿茶素(8)、芦丁(13)、槲皮素-3-O-(2″-O-没食子酰基)-β-D-吡喃葡萄糖苷(17)和槲皮素(18)与1/MIC关联度较好(>0.8)。结论:头花蓼抑菌作用是其多个化学成分共同作用的结果,没食子酸、表儿茶素、儿茶素、芦丁、槲皮素-3-O-(2″-O-没食子酰基)-β-D-吡喃葡萄糖苷和槲皮素是头花蓼发挥抑菌作用的主要物质基础。该研究结果为头花蓼及其相关产品的质量控制及深层次开发奠定了基础。
Objective: To clarify the material basis of the antibacterial effect of Polygonum capitatum. Methods: The components of Polygonum capitatum were prepared by D101 macroporous resin and MCI column chromatography. The components of Polygonum capitatum were established by UHPLC-UV technique. The main peaks were assigned by reference substance. The obtained components were formulated into samples with different peak compositions by uniform design. Their antibacterial activity was determined by examining the minimum inhibitory concentration (MIC) of each sample against Escherichia coli, Correlation analysis of 1 / MIC and chromatographic peak area correlation analysis, clear antibacterial activity-related major chromatographic peaks, to determine the inhibitory effect of Polygonum capitatum material basis. Results: The chromatographic peaks of gallic acid (1), epicatechin (6), catechin (8), rutin (13), quercetin -3-O- (2 “-O- galloyl) (17) and quercetin (18) had a good correlation with 1 / MIC (> 0.8) .Conclusion: The antibacterial activity of Polygonum capitatum is a result of the interaction of multiple chemical constituents. Acid, epicatechin, catechin, rutin, quercetin -3-O- (2 ”-O-galloyl) -β-D-glucopyranoside and quercetin The main material basis for the role of bacteria. The results laid the foundation for the quality control and further development of Polygonum capitatum and its related products.