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采用1/2半致死剂量的嗜水气单胞菌ZN1对鳗鲡进行腹腔注射,在第0 d、1 d、3 d、7 d、14 d、21 d采集鳗鲡肝、脾、肾、肠,观察鳗鲡经嗜水气单胞菌活菌攻击后各脏器的组织病理变化及脏器的损伤修复情况。结果显示,嗜水气单胞菌活菌ZN1攻击后鳗鲡肝、肾、脾、肠等组织细胞均出现程度不同的病变,且持续时间长达14 d以上,同时血液中的血栓细胞急剧减少,甚至全部消失。上述结果表明嗜水气单胞菌攻击后可引发鳗鲡各脏器发生明显的病理变化,并提示在病原菌攻击引起鳗鲡血栓细胞的减少并恢复缓慢可能是嗜水气单胞菌导致宿主产生败血症状的重要病理机制。
The eels were injected intraperitoneally with Z1 (1/2) lethal dose of Aeromonas hydrophila, and the liver, spleen, kidney, intestine, liver and intestine of eel were collected on day 0, day 1, day 3, day 7, day 14, day 21, Observed Eel î € "Aeromonas hydrophila attack organ histopathological changes and organ damage repair situation. The results showed that after the challenge with Aeromonas hydrophila ZN1, the pathological changes of liver, kidney, spleen, intestine and other tissues and cells of different degrees appeared in different degrees and lasted for more than 14 days. At the same time, the blood thrombus decreased sharply, Even all disappear. The above results indicate that Aeromonas hydrophila can induce significant pathological changes in various organs of Anguilla japonica after the challenge with Aeromonas hydrophila and suggest that the reduction of angiosperms and the slow recovery of Anguilla anguilla may be caused by Aeromonas hydrophila causing the host to produce sepsis Symptoms of the important pathological mechanism.