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目的介绍一种经济实用、成功率高的气管插管方法来制备矽肺大鼠模型。方法将20只SPF级Wistar雄性大鼠随机分为对照组和矽肺模型组,2组均使用自制实验器具通过直视下行插管法给药,经检验插管成功后向对照组气管注入1 ml生理盐水,模型组气管注入1 ml Si O2悬浊液。染毒后28 d取出肺组织行HE和Masson染色,应用碱水解法检测肺组织中羟脯氨酸含量。结果 2组大鼠造模过程中无死亡;造模后对照组大鼠生长状况良好,模型组大鼠生长缓慢并伴有毛枯、喘息等症状;肺组织病理学检查发现,对照组大鼠肺组织结构正常,无明显纤维化;模型组大鼠肺组织出现典型的肺泡炎和纤维化改变。模型组大鼠羟脯氨酸含量明显高于对照组,差异有统计学意义(P<0.01)。结论组织病理学检查及羟脯氨酸测定结果提示经改良的气管插管注药方法成功复制了矽肺模型,操作简便,死亡率低,可加以推广。
Objective To introduce an economical and practical method of tracheal intubation with high success rate to prepare silicotic rat model. Methods Twenty SPF Wistar male rats were randomly divided into control group and silicosis model group. Both groups were given self-made experimental instruments by direct intubation. After successful intubation, intratracheal injection of 1 ml Saline, model group tracheal injection of 1 ml Si O2 suspension. The lung tissues were removed at 28 days after exposure to HE and Masson staining, and hydroxyproline content in lung tissue was detected by alkaline hydrolysis. The rats in the control group grew well, the rats in the model group grew slowly and were accompanied by the symptoms of hairiness and wheezing. The pathological examination of the lung tissue showed that the rats in the control group Normal lung tissue structure, no significant fibrosis; model group rats lung typical alveolitis and fibrosis. The content of hydroxyproline in the model group was significantly higher than that in the control group, the difference was statistically significant (P <0.01). Conclusion The results of histopathological examination and hydroxyproline test indicate that the silicosis model was successfully replicated by the modified intratracheal intratracheal instillation. The method is simple and easy to operate with low mortality and can be extended.