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富勒烯纳米材料因其独特的结构和生物活性在生物医学领域广受关注,然而真正实现它们的商业化应用,仍需要进一步地研究,解决关键的生物安全性问题.本文利用电弧放电法,以稳定同位素13C直接替位掺入C60碳笼上的骨架碳原子,合成了13C-C60,确定了稳定同位素13C的掺入量,研究了稳定同位素效应所引起的C60分子质谱及振动光谱的变化特征,证实这种13C直接标记法不破坏富勒烯碳笼的本征结构.研究发现13C标记的C60分子的质谱分子离子峰呈正态分布,较强的分子离子峰m/z随13C标记量的增加而增大;其分子振动的红外和拉曼光谱虽然都保留了C60分子的特征光谱峰,但又随13C标记量的增加而发生不同程度地迁移和劈裂,这主要是由于稳定同位素13C的掺入,致使C60分子结构的对称性降低.这种稳定同位素13C标记富勒烯13C-C60,将为安全、无损、定量的富勒烯纳米材料体内的生物安全性研究奠定重要基础.
Due to their unique structure and biological activity, fullerene nanomaterials are attracting a great deal of attention in the field of biomedicine, however, their commercial applications still need to be further studied to solve the key biosafety issues.In this paper, arc discharge method, 13C-C60 was synthesized by direct substitution of stable isotope 13C for C60 carbon cage. The stable isotope 13C incorporation was determined. The change of C60 molecular mass spectrum and vibrational spectrum caused by stable isotope effect It is confirmed that this 13C direct labeling method does not destroy the intrinsic structure of the fullerene carbon cage.It is found that the molecular ion peaks of 13C labeled C60 molecules are normal distribution and the strong molecular ion peak m / Although the molecular vibrational infrared and Raman spectra retained the characteristic peaks of C60 molecules, they migrated and cleaved with different amounts of 13C, which were mainly attributed to the stability Incorporation of isotope 13C results in a reduction in the symmetry of the molecular structure of C 60. This stable isotope 13C-labeled fullerene 13C-C60 will provide a safe, non-destructive and quantitative fullerene nanomaterial in vivo Material safety research laid an important foundation.