【摘 要】
：
In searching for differentially expressed genes in human uterine leiomyomas (ULs), suppression sub-tractive hybridization was used to construct an UL up-regulat
【机 构】
：
State Key Laboratory of Molecular Biology
论文部分内容阅读
In searching for differentially expressed genes in human uterine leiomyomas (ULs), suppression sub-tractive hybridization was used to construct an UL up-regulated library, which turned out to represent 88genes. After two rounds of screening by reverse Northern analysis, twenty genes were proved to be up-regulated, including seventeen known genes and three genes with unknown function. All these genes werefirstly associated with UL. Three genes with notable difference were selected for Northern confirmationOur results proved the authenticity of the twenty genes. One gene named Phospholipase A2 (PLA2) showedup-regulation in 4/6 of the patients and investigation of tissue distribution indicated that it had obviousexpression in prostate, testis, liver, heart and skeletal muscle.
其他文献
[目的]调查沈阳地区老年慢性咳嗽(CC)患者特异性生活质量.[方法]选择本院呼吸内科就诊老年CC患者41例(观察组),入选对象均在沈阳地区生活5年以上,均在治疗前接受“莱斯特咳嗽
[目的]探讨联合检测基质金属蛋白酶-9(MMP-9)和白细胞介素6(IL-6)预测早产的价值.[方法]采用ELISA法测定40例孕28～36周先兆早产的孕妇(研究组)和40例同期同孕周的正常孕妇(对
用50～200 mmol/L NaCl处理2 d后,大麦(Hordeum vulgare L.)品种"滩引2号"(耐盐性强)根的液泡膜H+-ATPase活性增强,600 mmol/L NaCl处理下酶活性下降;"科品7号"(耐盐性弱)在50～1
为探讨应用转基因动物乳腺生物反应器高效表达人凝血因子IX(human clotting factor IX,hFIX)的可行性,构建了包括山羊β-酪蛋白基因启动子和外显子l、内含子1、外显子2共约6.
Histological characteristics of gametogenesis of two kinds of sca llops, gonochoric Chinese scallop, Chlamys farreri and hermaphroditic bay scallop, Argopecten
In order to investigate the indication of major pollen types to source plants, 39 surface pollen samples are collected from 39 plant communities. The results sh
A new species of cymothoid Isopoda, Nerocila donghaiensis , collected from the body of a sciaenid fish, Johnius sp., is described in the present paper.
对水蔗草(Apluda mutica L.)的生殖方式进行研究,结果表明水蔗草进行兼性无融合生殖.胚囊发育分为两种类型,即有性生殖的蓼型和无孢子生殖的大黍型.无融合生殖胚囊频率为60.7
GS系列环保型病理试剂是一套国内研制的甲醛和二甲苯替代试剂,它可以替代传统的以甲醛二甲苯为主体的石蜡切片技术流程,减少室内有毒有害气体的污染和毒害作用,同时杜绝大量
将萜烯类吲哚生物碱代谢关键酶--色氨酸脱羧酶（TDC）的编码基因转到烟草（Nicotiana tabacum L.)植物体内，标定在不同的亚细胞区室表达。通过蛋白免疫印迹法和色胺在植物体内的累积