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目的:探讨非小细胞肺癌(NSCLC)组织中EGFR、VEGF及Cbl蛋白表达及与临床病理特征的关系。方法:免疫组化SP法检测110例NSCLC石蜡标本和6例癌旁肺组织芯片EGFR、VEGF和Cbl蛋白表达,分析其与临床病理特征的关系。结果:110例肺癌组织中EGFR、VEGF和Cbl表达阳性率分别为58.2%(64/110),79.1%(87/110)和31.8%(35/110),其中EGFR、VEGF的表达高于癌旁正常肺组织,P值分别为0.007和0.03。体积小的肿瘤组织中Cbl表达增高,P=0.033。EGFR和VEGF呈正相关,P<0.01;在无淋巴结转移、<60岁患者中VEGF与Cbl呈正相关,P<0.05。肿瘤大小和淋巴结转移是影响预后的独立危险因素。结论:EGFR、VEGF在NSCLC组织中过表达,在肿瘤血管形成中起协同作用;Cbl可能具有泛素化肿瘤相关蛋白的作用。
Objective: To investigate the expression of EGFR, VEGF and Cbl protein in non-small cell lung cancer (NSCLC) and its relationship with clinicopathological features. Methods: The expressions of EGFR, VEGF and Cbl protein in 110 NSCLC paraffin specimens and 6 adjacent noncancerous lung tissues were detected by immunohistochemical SP method. The relationship between them and clinicopathological features was analyzed. Results: The positive rates of EGFR, VEGF and Cbl in 110 cases of lung cancer were 58.2% (64/110), 79.1% (87/110) and 31.8% (35/110), respectively Next to normal lung tissue, P values were 0.007 and 0.03, respectively. Small volume of tumor tissue Cbl expression increased, P = 0.033. There was a positive correlation between EGFR and VEGF (P <0.01). There was a positive correlation between VEGF and Cbl in patients <60 years old without lymph node metastasis (P <0.05). Tumor size and lymph node metastasis are independent prognostic risk factors. Conclusion: EGFR and VEGF are overexpressed in NSCLC tissue and play a synergistic role in tumor angiogenesis. Cbl may have the function of ubiquitination of tumor-associated proteins.