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目的 观察过量氟对实验大鼠骺板软骨和关节软骨Ⅱ和Ⅹ型胶原表型表达及细胞分化的影响 ,探讨过量氟对软骨发育的影响和氟骨症的发病机制。方法 6 0只SD大鼠 ,随机分为对照组 (19只 ,饮蒸馏水 )、低过量氟组 (2 0只 ,饮水氟浓度为 5 0mg/L) ,高过量氟组 (2 1只 ,饮水氟浓度为10 0mg/L)。实验 10周和 2 0周时 ,处死大鼠取胫骨关节软骨和骺板软骨 ,HE染色 ,Ⅱ、Ⅹ型胶原单克隆抗体免疫组织化学法测定软骨Ⅱ、Ⅹ型胶原表型表达变化。结果 随饮水中过量氟浓度的增高 ,大鼠胫骨骺板软骨增生层、肥大层细胞滞留 ,关节软骨出现散在软骨细胞坏死 ;过量氟大鼠软骨Ⅱ型胶原在骺板软骨滞留或增生过度的增生层和肥大层中表达 ,在关节软骨中表达减少 ;Ⅹ型胶原在滞留的肥大软骨细胞区 ,突入先期钙化带中的软骨半岛、软骨岛和骨化岛边缘表达 ;高过量氟组大鼠关节软骨表、中层Ⅹ型胶原表达增强。结论 过量氟对大鼠软骨细胞分化有两种结果 :四肢骺板软骨增生层、肥大层软骨细胞分化过度而滞留 ,加之基质矿化障碍 ,导致骨软化发生 ;高过量氟组大鼠关节软骨Ⅱ型胶原表型表达减少 ,Ⅹ型胶原表型表达增强 ,趋向骨关节病发生
Objective To observe the effect of excessive fluoride on phenotypic expression and cell differentiation of type Ⅱ and type Ⅹ collagen in epiphyseal plate in experimental rats and to explore the effect of excess fluoride on the development of cartilage and the pathogenesis of skeletal fluorosis. Methods Sixty SD rats were randomly divided into control group (19 rats, drinking distilled water), low excess fluoride group (20 rats, drinking water fluoride concentration of 50 mg / L), high fluoride group (21 rats, drinking water Fluorine concentration of 10 0mg / L). At 10 weeks and 20 weeks, rats were sacrificed to take the tibial articular cartilage and epiphyseal cartilage, and the changes of phenotypes of cartilage type Ⅱ and collagen type X were determined by HE staining and type Ⅱ and Ⅹ collagen monoclonal antibodies immunohistochemistry. Results With the increase of excessive fluoride concentration in drinking water, the cartilage hyperplasia and hypertrophic layer in the tibial epiphyseal plate were retained, and the articular cartilage appeared scattered chondrocyte necrosis. Excessive fluoride rat cartilage type Ⅱ collagen accumulated in epiphyseal plate cartilage or hyperplasia Layer and hypertrophic layer, and decreased in articular cartilage. Type X collagen expressed in the area of hypertrophic chondrocytes that stuck in the pre-calcified zone, cartilage island and cartilaginous island and the edge of ossification island. Cartilage, middle type X collagen expression increased. Conclusion Excessive fluoride has two effects on the differentiation of chondrocytes in rats: epiphyseal plate cartilage hyperplasia layer and hypertrophic layer chondrocyte hyperplasia and retention, combined with matrix mineralization disorder, resulting in osteomalacia. In high fluoride group, articular cartilage Ⅱ Collagen type phenotype decreased, type Ⅹ collagen phenotype increased, tend to osteoarthritis