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以C57BL/6J小鼠为实验材料,研究色钉菇粗多糖对多巴胺能神经元损伤的保护作用,并用体外实验方法测定其抗氧化活性。用MPTP(1-甲基-4-苯基-1,2,3,6-四氢吡啶)方法复制了帕金森病小鼠模型,模型复制前,预先给予小鼠100mg/kg、200mg/kg、400mg/kg不同剂量的色钉菇粗多糖,通过行为学检测方法和免疫组化技术观察小鼠的行为变化以及黑质中多巴胺能神经元的数量变化;用抑制小鼠肝脂质过氧化能力的方法测定色钉菇粗多糖的抗氧化活性。结果显示,预先给予200mg/kg和400mg/kg剂量的色钉菇粗多糖能显著地改善帕金森病模型小鼠的行为症状,并能显著地降低MPTP所致的小鼠多巴胺能神经元的凋亡,这可能与该多糖的抗氧化活性有关。提示色钉菇多糖有开发成防治帕金森病药物的应用前景。
C57BL / 6J mice were used as experimental materials to study the protective effect of crude polysaccharide from Dactylogluceae on the dopaminergic neuron injury. The anti-oxidative activity of the polysaccharides was tested in vitro. The mouse model of Parkinson’s disease was replicated by MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine). Before the model was replicated, mice were preliminarily given 100 mg / kg, 200 mg / kg , 400mg / kg different doses of crude polysaccharides Starch Mushroom, behavioral testing methods and immunohistochemistry to observe changes in behavior of mice and substantia nigra dopaminergic neurons in the amount of change; with inhibition of liver lipid peroxidation Ability to determine the anti-oxidative activity of the rough polysaccharides. The results showed that pretreatment with 200mg / kg and 400mg / kg Crude polysaccharide can significantly improve the behavioral symptoms in mice with Parkinson’s disease and significantly reduce the MPTP-induced mouse dopaminergic neuron apoptosis This may be related to the antioxidant activity of the polysaccharide. Prompt color mushroom polysaccharides have developed into Parkinson’s disease prevention and treatment of drug applications.