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目的 观察日本血吸虫SIEA2 6 2 8kDa抗原的抗雌虫生殖作用。方法用AcA柱层析纯化日本血吸虫早期胚卵中的SIEA和SIEA2 6 2 8kDa成分 ,并以此纯化产物免疫BALB/c小鼠。将小鼠分为三组 ,分别用SIEA、SIEA2 6 2 8kDa和免疫佐剂免疫动物 ,然后进行尾蚴攻击感染。 4 6天后剖杀动物 ,冲虫 ,然后作虫荷测定及虫卵分类计数。结果 SIEA2 6 2 8kDa免疫组雌虫子宫内减卵率为 5 4 .8% ,与对照组比较有极显著性差异 ;该实验组动物肝脏和小肠组织内的虫卵数比对照组分别降低了 4 8.0 7%和 77.4 1% ,且以成熟虫卵数的下降较为显著 ,分别降低了 83.6 %和 93.3% ;粪卵数的下降幅度最为显著 ,达87.2 6 %。结论 SIEA2 6 2 8kDa可作为一种抗卵胚发育及抗雌虫生殖的候选抗原分子。
Objective To observe the antifemale reproductive effects of Schistosoma japonicum SIEA2 6 2 8 kDa antigen. Methods The SIEA and SIEA2 6 2 8 kDa components in the early embryo of Schistosoma japonicum were purified by AcA column chromatography and BALB / c mice were immunized with the purified product. The mice were divided into three groups, animals were immunized with SIEA, SIEA2 6 28kDa and immunoadjuvant, respectively, and then infected with cercariae challenge. 4 6 days after the animals were killed, red worms, and then for the determination of insect burden and egg classification count. Results The intrauterine ovulation rate of females in SIEA2 6 2 8kDa group was 54.8%, which was significantly different from that in control group. The number of eggs in the liver and intestine of experimental group was lower than that in control group 4 8.0 7% and 77.4 1%, respectively, and the decrease of the number of mature eggs was more significant, which decreased by 83.6% and 93.3% respectively. The drop of fecal egg number was the most significant, reaching 87.26%. Conclusion SIEA2 6 2 8kDa can be used as a candidate antigens against egg embryo development and fecundity.