论文部分内容阅读
目的观察新风胶囊(XFC)对佐剂关节炎(AA)大鼠滑膜、肺组织自噬相关基因(ATG)、微管相关蛋白1轻链3Ⅱ(LC3-Ⅱ)和beclin 1的影响。方法将大鼠随机分为正常对照(NC)组、模型对照(MC)组、来氟米特(LEF)组、XFC组,每组10只,除NC组外均将Freund完全佐剂(0.1 m L/只)注射于每只大鼠右后足跖皮内以致炎,第10天在尾根部注射Freund完全佐剂0.05 m L加强免疫,复制AA模型。致炎后第13天给药。NC组、MC组给予生理盐水灌胃,每天1次;LEF、XFC组给予相应药物灌胃,每天1次,连续给药30 d。观察大鼠足跖肿胀度、关节炎指数、肺功能,ELISA检测血清细胞因子B细胞刺激因子(BAFF)、白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)、IL-4、IL-10表达,透射电镜观察滑膜、肺组织自噬小体,反转录PCR检测大鼠滑膜、肺组织ATG5、ATG7、ATG12 mRNA,Western blot法检测大鼠滑膜、肺组织LC3-Ⅱ、beclin 1的蛋白表达。结果 XFC组大鼠肺功能参数、血清IL-4、IL-10表达升高;滑膜、肺组织自噬体及自噬溶酶体较MC组增加;血清IL-1β、BAFF表达及滑膜组织ATG7、ATG12 mRNA降低,肺组织ATG5、ATG7 mRNA降低,ATG12 mRNA升高;滑膜组织、肺组织LC3-Ⅱ、beclin 1升高。结论 XFC通过调节AA大鼠滑膜、肺组织细胞自噬,改善肺功能。
Objective To observe the effect of Xinfeng Capsule (XFC) on the synovial membrane, the expression of ATG, LC3-Ⅱ and beclin 1 in adjuvant arthritis (AA) rats. Methods The rats were randomly divided into normal control (NC) group, model control (MC) group, leflunomide (LEF) group and XFC group, with 10 rats in each group. Freund complete adjuvant m L / mouse) were injected into the right hind paw skin of each rat to induce inflammation. On the 10th day, the rats were injected with Freund’s complete adjuvant (0.05 m L) to the tail of the tail to reinforce the AA model. The first 13 days after the administration of inflammation. The rats in NC group and MC group were administered with saline once a day, while the LEF and XFC groups were administered with the corresponding drugs once a day for 30 days. The degree of paw edema, arthritis index and lung function of rats were observed. Serum levels of cytokines B-cell stimulating factor (BAFF), interleukin 1β (IL-1β), tumor necrosis factor-α (TNF- 4, IL-10 expression, transmission electron microscopy synovium, lung autophagosome, reverse transcription PCR detection of synovial membrane, lung tissue ATG5, ATG7, ATG12 mRNA, Western blot detection of synovial membrane, lung tissue LC3-Ⅱ, beclin 1 protein expression. Results The lung function parameters, serum IL-4 and IL-10 levels in XFC group were significantly increased; the synovial membrane, autophagosomes and autophagy lysosomes in lung tissue were increased compared with MC group; the expression of IL-1β, BAFF and synovial membrane The ATG7 and ATG12 mRNA levels were decreased, ATG5 and ATG7 mRNA levels were decreased and ATG12 mRNA levels were elevated in the lung tissues. The levels of LC3-Ⅱ and beclin 1 in the synovium and lung tissue were increased. Conclusion XFC can improve the pulmonary function by regulating the autophagy of AA rats synovial membrane and lung tissue.