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在黄瓜幼苗子叶期及第一真叶期(叶横宽5cm时)用高温灭活后的黄瓜霜霉菌孢子囊悬液及黄瓜菌核病果组织(病果及菌丝)浸出液进行诱导接种,可使黄瓜植株产生对黄瓜霜霉病的抗病性。在自然病原激发病害试验中,经菌核病果浸出液诱导处理的植株,其病株率未降低,病叶率比ck降低29.87％,相对免疫效果为33.52％;经失活霜霉菌孢子囊悬液诱导处理的植株,其病株率比ck降低10.00％,病叶率降低43.68％,相对免疫效果为43.25％。在人工接种病原激发病害试验中,经茵核病果浸出液诱导的植株,其病株率比ck降低10.00％,病叶率降低37.14％,相对免疫效果为44.15％;经霜霉菌孢子囊悬液诱导处理的植株,病株率比ck降低30.00％,病叶率降低53.11％,相对免疫效果为1.48％。若只在子叶期进行一次诱导接种,其抗病性与ck无显著差异。
The seedlings of cucumber seedlings and the first true leaf stage (leaf width of 5cm) with high temperature inactivated cucumber downy mildew spore suspension and cucumber sclerotioric fruit tissue (diseased and mycelium) leachate induced inoculation, Cucumber plants can produce cucumber downy mildew disease resistance. In natural pathogen-induced disease test, the diseased plants did not reduce the diseased plants induced by sclerotium leaching solution, the diseased leaves rate was reduced by 29.87% compared with ck and the relative immune effect was 33.52% The plants treated with liquid showed that the diseased plants decreased by 10.00%, the diseased leaves decreased by 43.68% and the relative immune effect by 43.25%. In artificial inoculation pathogen-induced disease test, the strain induced by M. tuberosum showed a 10% lower rate of diseased plants than that of ck, a decrease of 37.14% and a relative immune effect of 44.14% The induced plants had a diseased rate of 30.00% lower than that of ck, a diseased leaf rate of 53.11% and a relative immune effect of 1.48%. If only in the cotyledon stage induction vaccination, the disease resistance and ck no significant difference.