采用ＲＮＡ斑点杂交分析方法，对４０例食管癌组织和相对应的正常粘膜组织中谷胱甘肽硫转移酶（ＧｌｕｔａｔｈｉｏｎｅＳｔｒａｎｓｆｅｒａｓｅ，ＧＳＴｓ）同功酶基因ＧＳＴπ、ＧＳＴα和ＧＳＴμ基因的表达情况进行研究，结果食管癌组织中ＧＳＴπ基因表达阳性率为７７．５％，在相应的正常粘膜组织中表达的阳性率为９０．０％。在食管癌组织中ＧＳＴα和ＧＳＴμ基因表达水平较低，表达阳性率分别为３５．０％和２０．０％，而正常粘膜组织中ＧＳＴα和ＧＳＴμ基因表达的阳性率分别为２０％和１５％，ＧＳＴｓ在癌组织中表达阳性率与正常粘膜组织相比，Ｐ＞０．０５。ＧＳＴπ基因表达阳性率明显高于ＧＳＴα和ＧＳＴμ基因，Ｐ＜０．０５。结果提示：ＧＳＴｓ同功酶基因在食管癌发生发展中基因转录水平增高，ＧＳＴπ是食管癌中ＧＳＴｓ同功酶存在的主要形式。 The glutathione S-transferase (GSTs) isoenzyme gene GST     , GSTα, and GSTμ genes in 40 esophageal cancer tissues and corresponding normal mucosal tissues were analyzed by RNA dot blot hybridization. The expression status of GST-π was found to be 77.5% in esophageal cancer tissues and 90.0% in corresponding normal mucosa tissues. The expression of GSTα and GSTμgenes was low in esophageal cancer tissues, and the positive rates were 35.0% and 20.0%, respectively. The positive rates of GSTα and GSTμgene expression in normal mucosal tissues The positive rates of GSTs in cancer tissues were 20% and 15%, respectively, compared with normal mucosal tissues, P>0.05. The positive rate of GSTπ gene expression was significantly higher than that of GSTα and GSTμ, P<0.05. The results suggest that the gene transcription level of GSTs isoenzyme gene in the development of esophageal cancer is increased, GST-π is the main form of GSTs isoenzyme in esophageal cancer.