论文部分内容阅读
目的:建立肾血管性高血压合并2型糖尿病大鼠复合模型,通过检测不同时段的血压、血糖来观察此模型的稳定性。方法:雄性SD大鼠先采用两肾一夹法(2K1C)并饮用1.5%盐水建立高血压大鼠模型,在此模型成模的基础上喂养高糖、高脂饮食4周后经腹腔注射链尿佐菌素(35 mg/kg),然后继续予高糖、高脂饮食饲养至实验结束,检测不同时间点血压、空腹血糖、空腹胰岛素、血脂、血清C-反应蛋白,计算胰岛素敏感指数,并观察一般情况对所建模型进行评价。结果:高血压合并糖尿病组大鼠成模率70%,其血压、血糖值达到成模标准,至实验结束时较稳定,分别与假手术组、空白对照组比较差异有统计学意义(P<0.05)。结论:成功复制高血压合并2型糖尿病大鼠模型,该模型经济、操作简单、成模率高、稳定性好。
OBJECTIVE: To establish a compound model of renal hypertensive rats with type 2 diabetes mellitus, and observe the stability of this model by measuring blood pressure and blood glucose at different time points. Methods: Male Sprague-Dawley rats were induced by 2K1C and 1.5% saline to establish hypertensive rats model. The model was fed with high glucose and high fat diet for 4 weeks and then injected intraperitoneally (35 mg / kg), then continue to high-sugar, high-fat diet until the end of the experiment to detect blood pressure at different time points, fasting blood glucose, fasting insulin, serum lipids, serum C-reactive protein, calculate the insulin sensitivity index, And observe the general situation of the model was evaluated. Results: The rate of forming blood pressure and blood glucose in model group was 70% and the model group was stable at the end of experiment. There was significant difference between the model group and the blank control group (P < 0.05). Conclusion: The rat model of type 2 diabetes mellitus complicated with hypertension is successfully replicated. This model is economical and easy to operate with high modulus and good stability.