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目的研究组织蛋白酶B(CB)在正常SD大鼠胰腺中的表达,为进一步研究应用CB抑制剂治疗急性胰腺炎提供形态学依据。方法应用免疫荧光组织化学双重标记技术,在激光共聚焦显微镜下观察CB在正常SD大鼠胰腺中的分布。结果 CB免疫反应阳性细胞分布在胰岛的A细胞、B细胞、D细胞及PP细胞,阳性物质分布于细胞质,细胞核阴性。结论 CB在SD大鼠胰腺的表达,提示CB可能在胰腺起着非常重要的作用。
Objective To investigate the expression of cathepsin B (CB) in the pancreas of normal SD rats and to provide morphological evidence for the further study of CB inhibitors in the treatment of acute pancreatitis. Methods The distribution of CB in pancreas of normal SD rats was observed under confocal laser scanning microscope by immunofluorescence histochemical double labeling. Results CB immunoreactive cells distributed in the islet A cells, B cells, D cells and PP cells, positive substances distributed in the cytoplasm, the nucleus was negative. Conclusion CB expression in the pancreas of SD rats suggests that CB may play a very important role in the pancreas.