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为检测增殖诱导配体(APRIL)在自身免疫病(SLE和RA)患者中的表达情况以及它与B细胞刺激因子(BAFF)表达、疾病预后、抗-dsDNA抗体之间的相关性。收集58名自身免疫病患者以及20名健康对照的外周血,用实时定量RT-PCR方法检测PBMC中APRIL和BAFF mRNA的表达,用ELISA方法检测血浆中APRIL和BAFF蛋白水平。同时,检测血浆中IgG、IgA、IgM、BF和抗-dsDNA抗体水平。结果同对照组相比,APRIL mRNA水平在自身免疫病患者中显著升高,与BAFF mRNA表达水平有相关性。与疾病初发组和治疗后缓解组相比,治疗后来缓解组患者APRIL mRNA表达显著升高。疾病组外周循环中BAFF蛋白显著升高,APRIL蛋白未见升高,APRIL和BAFF之间未见显著意义相关性,A-PRIL蛋白与抗-dsDNA和疾病活动度之间未见显著相关。检测APRIL mRNA含量是判断自身免疫病患者治疗情况和疾病预后的有用指标。循环中APRIL和BAFF蛋白的产生可能受到不同机制调节,在自身免疫病的发生、发展以及转归中所起的作用也不同,为研究APRIL的产生机制、表达水平和自身免疫病程度、早期诊断、预后等的关系打下基础。
To detect the expression of proliferative-inducing ligand (APRIL) in patients with autoimmune diseases (SLE and RA) and its association with B-cell stimulating factor (BAFF) expression, disease prognosis, and anti-dsDNA antibodies. Peripheral blood samples from 58 patients with autoimmune diseases and 20 healthy controls were collected. The expression of APRIL and BAFF mRNA in PBMCs was detected by real-time quantitative RT-PCR. The levels of APRIL and BAFF in plasma were detected by ELISA. Meanwhile, the levels of IgG, IgA, IgM, BF and anti-dsDNA antibodies in plasma were measured. Results Compared with the control group, the level of APRIL mRNA in patients with autoimmune diseases was significantly increased, which correlated with the expression of BAFF mRNA. Compared with the initial disease group and the remission group, APRIL mRNA expression in the remission group was significantly increased after treatment. There was no significant correlation between APRIL and BAFF in the disease group, but no significant correlation between A-PRIL protein and anti-dsDNA and disease activity. Detection of APRIL mRNA levels is a useful indicator of the treatment and prognosis of patients with autoimmune diseases. Circulating APRIL and BAFF protein production may be regulated by different mechanisms in the occurrence, development and prognosis of autoimmune diseases play a different role in order to study the mechanism of APRIL production, the level of expression and the degree of autoimmune disease, early diagnosis , Prognosis and other relations lay the foundation.