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目的研究可吸入颗粒物对大鼠心率变异性的影响及普萘洛尔的干预作用。方法36只SD大鼠,均为雄性,随机分为3组(实验组、对照组、普奈洛尔组),每组12只。实验组大鼠按25mg(1ml)/kg体重经气管内缓慢注入颗粒物生理盐水悬液进行染毒,每周染毒2次,连续染毒4周;对照组以生理盐水代替颗粒物悬液。普萘洛尔组:按25mg/kg剂量,经大鼠气管内注入颗粒物生理盐水悬液染毒,同时根据体重及饮水量调整普萘洛尔的用量,控制溶于饮用水中的普萘洛尔摄入量约为50mg/kg·d-1。大鼠麻醉后经皮下引导电极连续采集心电信号,计算SDNN和r-MSSD。结果实验组末次染毒之前和末次染毒之后30min时的心率变异性分别较首次染毒之前和首次染毒之后30min时明显下降(p<0.05);普萘洛尔组大鼠末次染毒之前和染毒之后30min时的心率变异性分别较首次染毒之前和染毒之后30min时有所下降,但差异无统计学意义。实验组末次染毒之前和染毒之后30min时的心率变异性较普萘洛尔组大鼠末次染毒之前和染毒之后30min时明显下降(p<0.05)。结论可吸入颗粒物能够导致心率变异性的降低,普萘洛尔具有拮抗可吸入颗粒物所致心率变异性降低的作用。
Objective To study the effect of inhalable particles on heart rate variability in rats and the effect of propranolol. Methods Thirty - six SD rats, all male, were randomly divided into 3 groups (experimental group, control group and propranolol group), 12 in each group. The rats in the experimental group were injected slowly with physiological saline solution of 25mg (1ml) / kg body weight through the endotracheal tube. The rats in the experimental group were exposed twice a week for 4 weeks. The rats in the control group were replaced with the normal saline suspension. Propranolol group: 25mg / kg dose, the rat tracheal injection of particles suspended in saline solution, while adjusting the amount of propranolol according to body weight and water intake, control dissolved in drinking water of propranolol Er intake of about 50mg / kg · d-1. After anesthesia in rats, the ECG signals were continuously collected through the subcutaneous guiding electrodes to calculate SDNN and r-MSSD. Results The heart rate variability of the experimental group before and 30 min after the last exposure was significantly lower than that before the first exposure and 30 min after the first exposure (p <0.05). Before the last exposure of the propranolol group And heart rate variability after 30min exposure were lower than those before the first exposure and 30min after exposure, respectively, but the difference was not statistically significant. The heart rate variability in the experimental group before and 30 min after the last exposure was significantly lower than that in the propranolol group before and 30 min after the last exposure (P <0.05). Conclusions Respirable particulate matter can lead to the decrease of heart rate variability. Propranolol has the effect of antagonizing the decrease of heart rate variability caused by inhalable particulate.