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目的研究大鼠大脑中动脉缺血再灌注后,梗死对侧皮层氨基丁酸A(GABAA)受体α2和α3亚基的表达变化。方法采用大鼠大脑中动脉阻塞(MCAO)再灌注模型,分为MCAO组(n=18)和假手术组(n=18),分别于术后7d、30d和6个月断头取脑,采用免疫组织化学技术检测各组中梗死对侧皮层GABAA受体亚基α2和α3的表达情况。结果 MCAO组大鼠与假手术组大鼠相比,在7d时梗死对侧亚基α2和α3在皮层各个脑区改变无明显差异(P>0.05);30d和6个月时,亚基α3在各个脑区均显著升高(P<0.05);30d时亚基α2在梗死对侧的Par1和Par2脑区表达增加(P<0.05);6个月时,梗死对侧亚基α2在Par1脑区表达增加而在Par2脑区表达降低(P<0.05)。结论大鼠大脑中动脉缺血再灌注后梗死塞对侧皮层GABAA受体亚基α2和α3发生了广泛而长效的变化,这种变化提示脑缺血后GABAA受体功能受损,GABAA受体的改变可能参与了缺血后突触的重塑和神经网络重建。
Objective To investigate the expression changes of GABAA receptor α2 and α3 subunits in the contralateral cortex after middle cerebral artery occlusion (MCAO) in rats. Methods The MCAO group was divided into MCAO group (n = 18) and sham operation group (n = 18). The rats were sacrificed on the 7th day, the 3rd day and the 6th month after the operation. Immunohistochemistry was used to detect the expression of GABAA receptor subunits α2 and α3 in the contralateral cortex in each group. Results Compared with sham-operated rats, there was no significant difference in cortex subfields α2 and α3 in cortex between MCAO group and sham operation group (P> 0.05). At 30 and 6 months, subunit α3 (P <0.05). At 30 days, subunit α2 was increased in Par1 and Par2 brain regions (P <0.05), and at 6 months, The brain area increased while decreased in Par2 brain area (P <0.05). Conclusion The long and narrow changes of GABAA receptor subunits α2 and α3 in contralateral cortex after middle cerebral artery occlusion (MCAO) in rats are extensive and long-term changes. These changes suggest that GABAA receptor function is impaired after cerebral ischemia, Changes in the body may be involved in the remodeling of the ischemic synapse and neural network reconstruction.