论文部分内容阅读
目的 观察降钙素基因相关肽(CGRP)、降钙素受体样受体(CRLR)、孤儿受体RDC 1(RDC 1)和受体 活性修饰蛋白(RAMPs)等,在正常大鼠中脑导水管周围灰质(PAG)的表达。 方法 用实时定量逆转录聚合酶链 式反应(RT PCR)技术,以大鼠β actin为内参,分别记录CGRP、CRLR、RAMP1、RAMP2、RAMP3和RDC 1mRNAsPCR 产物的CT值,计算每一样品对应其内参的相对含量。 结果 大鼠PAG区域,脑组织CGRP、CRLR、RAMP1和 RAMP2mRNAs均出现特异性DNA扩增产物。其中CGRP和RAMP1mRNAs表达水平较高,CRLRmRNA次之, RAMP2mRNA较弱;而RAMP3和RDC 1未出现特异性DNA扩增产物。 结论 大鼠PAG区域脑组织存在CGRP, CRLR,RAMP1和RAMP2mRNAs的特异性表达;它们共同参与生理过程的调节。
Objective To observe the effects of calcitonin gene related peptide (CGRP), calcitonin receptor - like receptor (CRLR), orphan receptor RDC 1 (RDC 1) and receptor activity modified proteins (RAMPs) Periaqueductal gray matter (PAG) expression. Methods The real-time RT-PCR technique was used to determine the CT value of PCR products of CGRP, CRLR, RAMP1, RAMP2, RAMP3 and RDC1 mRNA using rat β actin as internal reference. The relative content of internal reference. Results Specific DNA amplification products appeared in PAG region, brain CGRP, CRLR, RAMP1 and RAMP2 mRNAs. The expression of CGRP and RAMP1mRNAs were higher, followed by CRLRmRNA, and RAMP2mRNA was weaker. However, no specific DNA amplification products were found in RAMP3 and RDC1. Conclusion There are specific expressions of CGRP, CRLR, RAMP1 and RAMP2 mRNA in brain tissue of PAG rats. They all participate in the regulation of physiological processes.