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目的:建立测定复方银连颗粒中绿原酸含量的方法。方法:采用高效液相色谱法。色谱柱为Welchrom C18(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸(10∶90),检测波长为327nm;流速为1.0mL·min-1,柱温为30℃。结果:绿原酸进样量在0.3035～1.5175μg范围内与峰面积积分值呈良好线性关系(r=0.9996);平均回收率为101.6%,RSD=1.84%(n=6)。结论:本方法操作简便、可靠,可用于复方银连颗粒的质量控制。
Objective: To establish a method for determination of chlorogenic acid in Fufang Yinlian granules. Methods: Using high performance liquid chromatography. The column was Welchrom C18 (250mm × 4.6mm, 5μm). The mobile phase was acetonitrile-0.1% phosphoric acid (10:90). The detection wavelength was 327nm. The flow rate was 1.0mL · min-1. The column temperature was 30 ℃. Results: There was a good linear relationship between the injection volume of chlorogenic acid and peak area in the range of 0.3035-1.5175 μg (r = 0.9996). The average recovery was 101.6% and RSD was 1.84% (n = 6). Conclusion: The method is simple, reliable, and can be used for the quality control of Fufang Yinlian granules.