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该文研究了mi R-486在小鼠肝细胞内是否可通过调控其靶基因Pten(phosphatase and tensin homolog,同源性磷酸酶–张力蛋白)/Foxo1(forkhead box O1,叉头转录因子)进而影响甘油三酯(triglyceride,TG)和极低密度脂蛋白(very low density lipoprotein,VLDL)的合成。采用腺病毒载体感染小鼠肝癌细胞Hepa1-6,并测定靶基因Pten/Foxo1表达及VLDL和TG变化。腺病毒载体感染mi R-486的模拟剂(mimic)和抑制剂(antago)至细胞株Hepa1-6,Ad-mi R-486 mimic组mi R-486水平显著增加(P<0.001),PTEN/Fox O1的m RNA和蛋白质水平显著降低,细胞内VLDL含量显著降低(P<0.01),TG含量明显增加(P<0.05);反之,Ad-mi R-486 antago组mi R-486表达水平受到抑制,PTEN/Fox O1的m RNA和蛋白质水平显著增加(P<0.01),且细胞内VLDL含量显著增加(P<0.01),TG含量明显降低(P<0.05)。该研究结果表明,mi R-486可能通过调节Pten/Foxo1来影响细胞内的VLDL和TG生成。
This study investigated whether mi R-486 can regulate the target gene Pten (phosphatase and tensin homolog) / Foxo1 (forkhead transcription factor) in mouse hepatocytes Affects the synthesis of triglyceride (TG) and very low density lipoprotein (VLDL). Hepatoma cell Hepa1-6 was infected with adenovirus and the expression of Pten / Foxo1, VLDL and TG were detected. The mi R-486 level of mi R-486 mimic and antago cells was significantly increased (P <0.001) in Ad-mi R-486 mimic group and the expression of PTEN / The mRNA and protein levels of FoxO1 were significantly decreased, the content of VLDL in cells was significantly decreased (P <0.01) and the content of TG was significantly increased (P <0.05). On the contrary, the expression of mi R-486 in Ad-mi R- (P <0.01). The content of VLDL in cells was significantly increased (P <0.01) and the content of TG was significantly decreased (P <0.05). The results of this study indicate that mi R-486 may affect VLDL and TG production in cells by regulating Pten / Foxo1.