A new kinetic spectrophotometric method for the determination of major metabolite of heroin in biolo

来源 :Chinese Chemical Letters | 被引量 : 0次 | 上传用户:talenthers312
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
This study describes a simple,rapid and selective catalytic kinetic spectrophotometric method for the determination of 6- monoacetylmorphine(6-MAM) as major metabolite of heroin in biological samples.The method is based upon the catalytic effect of 6-MAM on the oxidation of Janus Green by bromate in acid media.The reaction was followed spectrophotometrically by measuring the decrease in absorbance of Janus Green at 618 nm.The dependence of sensitivity on the reaction variables was studied.Under optimum conditions,two linear calibration curves over the range 0.1-1.0μg mL~(-1) and 1.0-34.0μg mL~(-1) of 6- MAM were obtained.The detection limit was 1.2×10~2μg mL~(-1) of 6-MAM.The relative standard deviations for six replicate determinations of 0.8 and 5.0μg mL~(-1) of 6-MAM were 1.4 and 1.1%respectively.The effect of various species commonly associated with heroin in real samples was also investigated.The proposed method was successfully applied in human urine and serum samples with satisfactory results. This study describes a simple, rapid and selective catalytic kinetic spectrophotometric method for the determination of 6- monoacetylmorphine (6-MAM) as major metabolite of heroin in biological samples. The method is based upon the catalytic effect of 6-MAM on the oxidation of Janus Green by bromate in acid media. The reaction was followed spectrophotometrically by measuring the decrease in absorbance of Janus Green at 618 nm. The dependence of sensitivity on the reaction variables was studied. Unitized optimum conditions, two linear calibration curves over the range 0.1- 1.0μg mL -1 and 1.0-34.0μg mL -1 of 6-MAM were obtained. The detection limit was 1.2 × 10 ~ 2μg mL -1 of 6-MAM.The relative standard deviations for six replicate determinations of 0.8 and 5.0 μg mL -1 of 6-MAM were 1.4 and 1.1% respectively. The effect of various species commonly associated with heroin in real samples was also investigated.The proposed method was successfully applied in human urine and serum samples with s atisfactory results.
其他文献
银行及金融业对数据的真实性及安全可靠性要求都及其严格.作为数据的载体——数据库的一致性,稳定性以及性能和安全性都必须满足要求才能进入金融行业.长期以来以 ORACLE、DB
期刊
对赌协议是商业投资领域的一种普遍的法律文件,其在国外成熟资本市场早已被广泛应用,大大降低了交易成本,同时也提高了投资的效率.而我国的法律体系和市场环境都是和国外有着
当今社会,电子商务迅猛发展,网络购物成为成为一种便利方式.与此同时,网络交易中消费者权利面临着许多问题,网络交易尤其自身的特点,消费者自己的个人隐私、联系方式、购物习
高检院出台的监所检察四个办法对于服刑罪犯又犯罪案件的办理有了明确的规定.这里对于监狱内以及看守所内留所服刑罪犯又犯罪案件由相对应的检察机关刑事执行检察部门办理是
有限合伙制度作为一种古老的商事组织形态,因其独特的责任设计模式和相对简单的组织结构,弥补了公司制与合伙制之间的制度空白.本文通过对有限合伙的起源与发展、概念、特征
在校企双育人主体中,双师型导师在人才培养过程中,起到非常关键的作用.校内专职教师的培养,学校育人主体有着完整的培养体系.企业兼职导师带徒弟,企业基本上没有完整激励制度
100年前,在哈佛大学的一个实验室里,一名年轻的学生正忙于观察一对对近亲交配的老鼠。当时,这个在校园里广为人知的“鼠人”的克拉伦斯·C·利图尔(Clarence Cook Little),正
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7
中小企业在中国经济运行体制当中之中发挥着重要作用,包含国家经济速度的提高、减轻工作重担等.当下一些中小企业在平时的运营过程之中还是或多或少的存在一些问题,所以,尤其
期刊
当前,学界围绕狐这一主题已着墨甚多,狐作为文学上的一种独特意象,是反映当时的社会文化、宗教信仰、审美意识等的一种文学意象。本文将通过分析《搜神记》和《今昔物语集》
期刊