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目的:建立丹黄祛瘀片中黄芪甲苷的含量测定方法。方法:采用HPLC-UV,色谱柱为Kromasil C18柱(250 mm×4.6mm),流动相为乙腈-水(32∶68),流速1.0mL/min,检测波长203nm。结果:黄芪甲苷在0.066～0.330mg/mL范围内线性关系良好(r=0.9993),回收率在95.0%～105.0%之间,平均回收率为100.1%(RSD=2.0%,n=9)。结论:该法简便,快速,结果准确,重复性好,可用于控制丹黄祛瘀片中黄芪甲苷的含量。
Objective: To establish a method for the determination of Astragaloside Ⅳ in Danhuang Quyu tablets. Methods: HPLC-UV was used. The column was Kromasil C18 (250 mm × 4.6 mm). The mobile phase was acetonitrile-water (32:68) with a flow rate of 1.0 mL / min and the detection wavelength was 203 nm. Results: The calibration curve of astragaloside Ⅳ was linear in the range of 0.066-0.330 mg / mL (r = 0.9993). The average recoveries were between 95.0% and 105.0% (RSD = 2.0%, n = 9) . Conclusion: The method is simple, rapid, accurate, reproducible and can be used to control the content of Astragaloside IV in Danhuang Quyu tablets.