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目的探讨新疆目前流行的麻疹病毒的基因型别和特征。方法采用Vero/SLAM细胞分离培养麻疹病毒,通过反转录-聚合酶链反应方法扩增麻疹病毒N基因羧基末端456 bp片段,并对PCR产物进行序列测定和同源性分析。结果 2013 2014年新疆分离到7株麻疹病毒均为H1a基因亚型,与Shanghai-191同源性为89.5%~87.3%;与世界卫生组织推荐用于鉴定基因型别的6株H1基因型同源性为91.0%~99.3%,与目前3株流行H1a亚型同源性为97.3%~100.0%。结论 H1a基因亚型仍为新疆本土流行病毒株的优势亚型,近年来引起新疆麻疹流行的麻疹病毒基因序列存在着不同程度的差异,可能来自于不同的传播链,麻疹病毒株H1a亚型的持续传播与易感人群的累积和人口流动有密切关系。
Objective To explore the genotypes and characteristics of the measles virus currently prevailing in Xinjiang. Methods The measles virus was isolated and cultured in Vero / SLAM cells. The 456 bp fragment of carboxyl terminal of measles virus N gene was amplified by reverse transcription - polymerase chain reaction (PCR). The PCR products were sequenced and analyzed for homology. Results The seven isolates of measles virus isolated in Xinjiang in 2014 were all H1a genotypes, which shared 89.5% -87.3% homology with Shanghai-191. The six H1 genotypes recommended by WHO for genotyping Their homology was 91.0% -99.3% with the prevalence of the three strains of H1a at present ranged from 97.3% to 100.0%. Conclusion H1a subtype is still the predominant subtype of local endemic strains in Xinjiang. In recent years, the genetic diversity of measles virus that causes the epidemic of measles in Xinjiang may differ to some extent. It may originate from different transmission chains. The subtype H1a of measles virus strain The spread of persistent and susceptible populations is closely linked to population movements.