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本文报道一个专属、准确、精密、灵敏的抗癫痫药物苯妥英(Phenytoin DPH)、卡马西平(Carbamazepine,CBZ)和苯巴比妥(Phenobarbital,PB)血药浓度的反相高效液相色谱测定法。本法以5—乙基—5—对甲苯基巴比妥酸为内标,采用简单的溶液提取,于40℃、氮气流蒸干,流动相制成供试液进样。以乙腈、甲醇、水和氯仿(10∶35∶56∶0.13,V/V)为流动相,于254nm检测。在三个不同水平,DPH、CBZ、PB的平均回收率为97.81%、98.14%和100.7%(n=12),日内和日间变异均低于5%。内源性物质及34种常用药物未见干扰本测定条件,在分析范围内具有良好的线性关系,并已在临床分析中应用。
This article reports an RP-HPLC method for the determination of the concentrations of Phenytoin DPH, Carbamazepine (CBZ) and Phenobarbital (PB), an exclusive, accurate, sensitive and sensitive antiepileptic drug. . In this method, 5-ethyl-5-p-tolylbarbituric acid is used as an internal standard. The solution is extracted by a simple solution and evaporated to dryness under a nitrogen stream at 40 ° C. The mobile phase is prepared for sample injection. The mobile phase was acetonitrile, methanol, water and chloroform (10:35:56:0.13, V / V) and detected at 254nm. At three different levels, the average recoveries for DPH, CBZ and PB were 97.81%, 98.14% and 100.7% (n = 12) with daily and intra-day variability of less than 5%. Endogenous substances and 34 kinds of commonly used drugs did not interfere with the determination of the conditions in the analysis range has a good linear relationship and has been used in clinical analysis.