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目的 研究人类乳头瘤病毒 (HPV) 6、11、16和 18型及P5 3与膀胱移行细胞癌的关系。方法 采用聚合酶链反应 (PCR)方法检测了 75例膀胱移行细胞癌组织中HPV的感染 ,免疫组化SP法检测P5 3蛋白表达情况。结果 膀胱移行细胞癌组织中HPV6、11、16和 18的阳性率分别为 6 .7%(5 75 ) ,5 .3 % (4 75 ) ,33 .3 % (2 5 75 )和 6 .7% (5 75 )。低危型HPV(6或 11)阳性率为 9.3% (7 75 ) ,高危型HPV(16或 18)阳性率为 34.7% (2 6 75 )。同一膀胱癌组织中两种以上 (包括两种 )HPV亚型感染 8例 ,占 10 .6 %。HPV6、16和 18型之间感染阳性率在肿瘤有无转移组中差异显著 (P <0 .0 5 ) ,HPV16、18的阳性率在肿瘤病理分级中差异有极显著性 (P <0 .0 1)。HPVDNA型别的分布在膀胱癌中以 16型为主。P5 3蛋白在膀胱移行细胞癌组织中的阳性表达率为 44 %。结论 高危型HPV感染与膀胱癌发生有关
Objective To study the relationship between human papillomavirus (HPV) types 6, 11, 16 and 18 and P5 3 and bladder transitional cell carcinoma. Methods The HPV infection in 75 cases of bladder transitional cell carcinoma was detected by polymerase chain reaction (PCR). The expression of P53 protein was detected by immunohistochemical SP method. Results The positive rates of HPV6, 11, 16 and 18 in transitional cell carcinoma of the bladder were 6.7% (5 75), 5.3% (4 75), 33 3% (25 75) and 6.7 % (5 75). The positive rate of low-risk HPV (6 or 11) was 9.3% (7 75), and the high-risk HPV (16 or 18) was 34.7% (2 6 75). In the same bladder cancer more than two (including two) HPV subtype infection in 8 cases, accounting for 10.6%. The positive rates of HPV16, HPV16, HPV16 and HPV18 were significantly different between the groups with or without tumor metastasis (P <0.05). The positive rates of HPV16 and HPV18 in the pathological grading of tumors were significantly different (P <0.05). 0 1). HPVDNA type distribution in bladder cancer in the 16-based. P5 3 protein in transitional cell carcinoma of the bladder in the positive expression rate was 44%. Conclusion High-risk HPV infection is associated with bladder cancer