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目的观察60KD精子膜抗原对抗体阳性精子顶体酶活性的影响。方法用全胶电洗脱结合连续电洗脱的方法提取60KD精子膜抗原,使用60KD精子膜抗原体外处理抗精子抗体阳性精子。精子顶体酶活性的检测采用固定明胶底物薄膜法。结果抗精子抗体阳性精子经60KD抗原处理后,精子的凝集率由处理前的(35.115.1)%下降到了(13.03.3)%(P<0.001)。精子顶体酶阳性反应率由处理前的(30.37.3)%升高到(56.49.1)%(P<0.001)。结论60KD精子膜抗原可以有效去除精子结合的抗体,并提高精子的顶体酶活性。
Objective To observe the effect of 60 kD sperm membrane antigen on acrosin activity of acrosin positive sperm. Methods 60KD sperm membrane antigen was extracted by using all gel electrophoresis combined with continuous electroelution. Anti-sperm antibody positive sperm were treated with 60KD sperm membrane antigen in vitro. Sperm acrosin activity was measured using a fixed gelatin substrate membrane method. Results The sperm agglutination rate decreased from (35.115.1)% to (13.03.3)% before treatment (P <0.001) after anti-sperm antibody-positive sperm were treated with 60KD antigen. The sperm acrosin positive rate increased from (30.37.3)% before treatment to (56.49.1)% (P <0.001). Conclusion 60KD sperm membrane antigen can effectively remove sperm-bound antibodies and increase sperm acrosin activity.