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以血叶兰的嫩茎段为外植体,建立了血叶兰的组培快繁体系。结果表明:芽诱导最适宜的培养基是MS+6-BA 5.0mg·L-1;最适芽增殖培养基为MS+6-BA 5.0 mg·L-1+NAA 0.5 mg·L-1+TDZ 0.3 mg·L-1,芽增殖率达4.92倍;最佳壮苗培养基为MS+NAA 0.3 mg·L-1+GA3 1.0 mg·L-1+15%椰汁,芽苗高度达到4.33 cm,芽粗为0.61 cm;生根最适培养基为1/2MS+IBA 1.0mg·L-1+15%香蕉+0.5 g·L-1活性炭,生根率在93.0%以上;炼苗后,移栽在泥炭土+珍珠岩+松树皮(3:1:1,V/V/V)混合基质中,存活率高于87.0%。
With the tender stem segments of Heteropalaceae as explants, a tissue culture system was established. The results showed that the most suitable medium for bud induction was MS + 6-BA 5.0 mg · L -1, and the optimum culture medium for buds was MS + 6-BA 5.0 mg · L -1 + NAA 0.5 mg · L -1 + TDZ 0.3 mg · L-1, the bud proliferation rate was 4.92 times. The best seedling culture medium was MS + NAA 0.3 mg · L -1 + GA3 1.0 mg · L -1 +15% coconut milk, the bud height reached 4.33 cm and shoot diameter was 0.61 cm. The optimum medium for rooting was 1 / 2MS + IBA 1.0 mg · L-1 + 15% banana +0.5 g · L-1 activated carbon. The rooting rate was above 93.0% The survival rate was higher than 87.0% in a mixed medium of peat soil, perlite and pine bark (3: 1: 1, V / V / V)