论文部分内容阅读
目的研究毛郁金醇提物对大鼠心肌缺血再灌注损伤(MI/RI)的保护作用。方法将60只健康♂SD大鼠随机分为假手术组(给予等体积生理盐水)、模型组(给予等体积生理盐水)、复方丹参片组(CDT组,600 mg·kg~(-1))、毛郁金醇提物Ⅰ、Ⅱ、Ⅲ组(75、150、300 mg·kg~(-1)),将各组大鼠平行ig给药预处理7 d后,采用结扎冠状动脉左前降支30 min,松开结扎再灌注180 min复制MI/RI模型,然后于腹主动脉采血,测定血清中总抗氧化能力(TAC)、丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)、谷胱甘肽过氧化物酶(GSH-Px)、MB型肌酸激酶(CK-MB)及乳酸脱氢酶(LDH)的含量。结果与MI/RI模型组比较,经毛郁金醇提物预处理可以显著提高大鼠血清中TAC、SOD、GSH-Px和NO的水平,有效地抑制大鼠心肌中LDH及CK-MB的漏出,降低MDA的含量,实验表明:高剂量的效果最好,大部分指标接近于正常水平。结论毛郁金醇提物对大鼠MI/RI有良好的保护作用,并具有剂量依赖性,高剂量优于阳性药CDT,其主要作用机制可能与减少自由基聚集、提高抗氧化酶活力的作用有关。
Objective To study the protective effect of alcohol extract of Vitis amuletus on myocardial ischemia-reperfusion injury (MI / RI) in rats. Methods Sixty healthy male SD rats were randomly divided into sham operation group (given equal volume of normal saline), model group (given equal volume of normal saline), compound Danshen Tablet group (CDT group, 600 mg · kg -1) ), Ethanol extract of Melastoma villosum (group Ⅰ, Ⅱ, Ⅲ) (75,150,300 mg · kg -1). After pretreatment with parallel ig administration for 7 days, the left anterior descending coronary artery was ligated The MI / RI model was replicated 180 min after ligation and reperfusion, and the blood samples were taken from abdominal aorta for determination of total antioxidant capacity (TAC), malondialdehyde (MDA), superoxide dismutase (SOD) Nitric oxide (NO), glutathione peroxidase (GSH-Px), creatine kinase type MB (MB-MB) and lactate dehydrogenase (LDH) Results Compared with the MI / RI model group, pretreatment with alcohol extract of S. miltiorrhiza could significantly increase the levels of TAC, SOD, GSH-Px and NO in the serum of rats and effectively inhibit the leakage of LDH and CK-MB in rat myocardium, Reduce MDA content, experiments show that: the best effect of high doses, most of the indicators close to normal levels. Conclusion Ethanol extract of S. miltiorrhiza has good protective effect on MI / RI in rats and its dose-dependent and high-dose is better than that of CDT. The main mechanism may be related to the reduction of free radical accumulation and the increase of antioxidant enzyme activity .