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OBJECTIVE:To investigate the influence of Yin-tonification herbal formulas Jaeumganghwa-tang (Ziyin Jianghuo Tang,JEGHT),Ssanghwa-tang (Shuanghe Tang,SHT) and Yukmijihwang-tang (Liuwei Di huang Tang,YMJHT) on the activities of human major cytochrome P450 (CYP450s) and UDP-glucuronosyltransferases isozymes (UGTs) in vitro.METHODS:The activities of CYP450s (CYP1A2,CYP3A4,CYP2B6,CYP2C9,CYP2C19,CYP2D6 and CYP2E1) and UGTs (UGT1A1,UGT1A4 and UGT2B7)were assessed using in vitro fluorescence-and luminescence-based enzyme assays,respectively.The effects of herbal formulas on the activities of CYP450s and UGTs were presented as ICs0 values.RESULTS:JEGHT showed the potent inhibition of the CYP2D6 activity,with weak inhibition on the activities of CYP1A2,CYP2B6,CYP2C9,CYP2C19,CYP2E1,CYP3A4,UGT1A1,UGT1A4 and UGT2B7.SHT inhibited the activities of CYP1A2 and CYP2E1,whereas the negligible inhibition of the activities of CYP2B6,CYP2C9,CYP2C19,CYP2D6,CYP3A4,UGT1A1,UGT1A4 and UGT2B7 through SHT was observed.YMJHT inhibited CYP2E1 activity,with a negligible inhibition on the activities of CYP1A2,CYP2B6,CYP2C9,CYP2C19,CYP2D6,CYP3A4,UGT1A1,UGT1A4 and UGT2B7.CONCLUSION:These findings provide information about the potential interactions between three Yin-tonification herbal formulas and conventional drugs.